IMAGING CORE

成像核心

• 批准号: 7720502
• 负责人: GRAZYNA RAJKOWSKA
• 金额: $ 10.22万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720502
• 关键词: 3-Dimensional; Animals; Area; Arts; Autopsy; Behavior; Brain; Brain region; California; Cell Count; Chromosome Pairing; Complement component C1s; Computer Retrieval of Information on Scientific Projects Database; Computer software; Condition; Confocal Microscopy; D Cells; Equipment; Fluorescence; Fluorescent Antibody Technique; Funding; Grant; Head; Histology; Human; Human Resources; Image; Image Analysis; Imagery; Immunohistochemistry; Institution; Instruction; Laboratories; Lasers; Light Microscope; Maintenance; Measurement; Medical center; Mental disorders; Methods; Microscope; Microscopic; Microscopy; Mississippi; Molecular; Morphology; Neuroanatomy; Neuroglia; Neurons; Neurosciences Research; Paper; Procedures; Process; Proteins; Psychiatry; Publishing; Research; Research Personnel; Resources; Sampling; Scanning; Slice; Source; Staining method; Stains; Supervision; Synapses; System; Techniques; Tissues; Training; United States National Institutes of Health; Universities; abstracting; brain tissue; cellular imaging; cryostat; density; design; digital; experience; insight; investigator training; laser capture microdissection; neurochemistry; neuropathology; professor; receptor binding

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As is the case for all areas of neuroscience research, the application of modern methods of cellular imaging are greatly accelerating advancements in the field. The center's Imaging Core gives investigators access to state-of-the-art, sophisticated microscopic equipment with advanced capabilities in the visualization, quantification and interpretation of neuronal systems at the cellular level. Dr. Grazyna Rajkowska, (Professor of Psychiatry and Human Behavior; Head of the Quantitative Neuroanatomy Laboratory), serves as Core Director of the CPN's Imaging Core. Dr. Rajkowska is a neuroanatomist with 20 years experience in quantitative image analysis of specific types of neurons and glial cells in human postmortem and animal brain tissues. She has served as Head of the Laboratory of Quantitative Neuroanatomy at UMC for the past 13 years and has published over 100 scientific papers and abstracts in the field of quantitative morphology. Dr. Rajkowska provides oversight of investigator training in the use of core equipment, the maintenance of core equipment, and the supervision of core personnel. In addition to the administrative oversight of the Imaging Core, Dr. Rajkowska instructs Center investigators and other UMC researchers in the general use of the microscopic imaging equipment and further offers highly specialized expertise in the technical parameters relevant to the design, application and interpretation of quantitative morphological and neurochemical studies. These technical parameters include tissue slicing and general histological staining of human postmortem and animal brain tissues, the application of 3-dimensional cell counting techniques to different types of staining procedures, the determination of optimal conditions for immunohistochemistry and guidance in the anatomical localization of the specific brain regions being studied. Dr. Jose Javier Miguel-Hidalgo (Assistant Professor of Psychiatry & Human Behavior, University of Mississippi Medical Center) is a neuroanatomist with 18 years experience in microscopy, histology and histochemical processing of brain samples in a variety of vertebrate species. Dr. Miguel-Hidalgo received formal training in confocal microscopy in 1992 at the University of California at Davis. CPN and UMC investigators often use confocal microscopy for double immunostaining with fluorescent antibodies. Dr. Miguel-Hidalgo also received instruction directly from Arcturus technical field trainers in 2005 and has subsequently trained several researchers at UMC using laser capture microdissection in their research. He further assists Dr. Rajkowska in determining the technical parameters (tissue sectioning, measurement, sampling, etc.) that would produce the best images for each investigator's particular application. The CPN's Imaging Core and UMC currently support a Imaging Facility providing shared use of the following imaging equipment: + Nikon C1 Laser Scanning Confocal Microscope + Arcturus Laser Capture Microdissection System with fluorescence and 60X objective + Kodak Imaging Station + Fuji BAS-5000 Phosphor Image Analysis System + MCID Image Analysis Systems (2) + AIS Image Analysis Workstation + StereoInvestigator and Neurolucida Quantitative Cell Counting System + 3-D Cell Counting System (Williams & Rakic) + Bioquant Cell Counting and Imaging System + Nikon Light Microscope, with stereoinvestigator software + Leica Stereomicroscope + Zeiss Axioskop Microscopes (2) + Axioskop Digital Photomicroscopy Workstation. + Leica Cryostat These sophisticated imaging systems give investigators the ability to estimate, in three-dimensional space, numbers of cells, terminals, or synapses and to analyze the density of receptor binding and the level of specific proteins, giving researchers unprecedented insight into the cellular and molecular neuropathology of mental disorders.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 就像神经科学研究的所有领域一样，现代细胞成像方法的应用正在极大地加速该领域的进步。该中心的成像核心使研究人员能够使用最先进、复杂的显微设备，这些设备具有在细胞水平上对神经系统进行可视化、量化和解释的先进能力。 Grazyna Rajkowska博士(精神病学和人类行为学教授，定量神经解剖学实验室负责人)担任CPN成像核心的核心主任。Rajkowska博士是一名神经解剖学家，在人类身体和动物脑组织中特定类型的神经元和神经胶质细胞的定量图像分析方面拥有20年的经验。在过去的13年里，她一直担任UMC定量神经解剖学实验室的负责人，发表了100多篇定量形态学领域的科学论文和摘要。Rajkowska博士负责监督调查员在使用核心设备、维护核心设备和监督核心人员方面的培训。 除了对成像核心的行政监督外，Rajkowska博士还指导中心调查人员和其他UMC研究人员使用显微成像设备，并进一步提供与定量形态和神经化学研究的设计、应用和解释相关的技术参数方面的高度专业化的专业知识。这些技术参数包括人死后和动物脑组织的组织切片和一般组织学染色，三维细胞计数技术在不同类型染色程序中的应用，免疫组织化学最佳条件的确定，以及对正在研究的特定脑区的解剖定位的指导。 何塞·哈维尔·米格尔-伊达尔戈博士(密西西比大学医学中心精神病学和人类行为助理教授)是一位神经解剖学家，在各种脊椎动物脑组织样本的显微镜、组织学和组织化学处理方面拥有18年的经验。米格尔-伊达尔戈博士于1992年在加州大学戴维斯分校接受了共焦显微镜的正式培训。CPN和UMC的调查人员经常使用共聚焦显微镜进行荧光抗体的双重免疫染色。Miguel-Hidalgo博士还在2005年直接接受了Arcturus技术领域培训师的指导，随后在UMC的研究中使用激光捕获显微解剖对几名研究人员进行了培训。他还协助Rajkowska博士确定技术参数(组织切片、测量、采样等)。这将为每个调查员的特定应用产生最佳图像。 CPN的成像核心和联华电子目前支持一个成像设施，提供以下成像设备的共享使用： +尼康C1激光扫描共聚焦显微镜 +Arcturus带荧光和60X物镜的激光捕获显微切割系统 +柯达成像站 +富士BAS-5000荧光粉图像分析系统 +MCID图像分析系统(2) +AIS图像分析工作站 +StereoInvestigator和NeuroLucida定量细胞计数系统 +3-D细胞计数系统(Williams&Rakic) +BioQuant细胞计数和成像系统 +尼康光学显微镜，配有立体声调查器软件 +徕卡立体显微镜 +蔡司Axioskop显微镜(2) +Axioskop数字光学显微镜工作站。 +徕卡低温恒温器 这些复杂的成像系统使研究人员能够在三维空间中估计细胞、终末或突触的数量，并分析受体结合的密度和特定蛋白质的水平，使研究人员能够前所未有地深入了解精神障碍的细胞和分子神经病理。