IMAGING CORE

成像核心

• 批准号: 7720502
• 负责人: GRAZYNA RAJKOWSKA
• 金额: $ 10.22万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720502
• 关键词: 3-Dimensional; Animals; Area; Arts; Autopsy; Behavior; Brain; Brain region; California; Cell Count; Chromosome Pairing; Complement component C1s; Computer Retrieval of Information on Scientific Projects Database; Computer software; Condition; Confocal Microscopy; D Cells; Equipment; Fluorescence; Fluorescent Antibody Technique; Funding; Grant; Head; Histology; Human; Human Resources; Image; Image Analysis; Imagery; Immunohistochemistry; Institution; Instruction; Laboratories; Lasers; Light Microscope; Maintenance; Measurement; Medical center; Mental disorders; Methods; Microscope; Microscopic; Microscopy; Mississippi; Molecular; Morphology; Neuroanatomy; Neuroglia; Neurons; Neurosciences Research; Paper; Procedures; Process; Proteins; Psychiatry; Publishing; Research; Research Personnel; Resources; Sampling; Scanning; Slice; Source; Staining method; Stains; Supervision; Synapses; System; Techniques; Tissues; Training; United States National Institutes of Health; Universities; abstracting; brain tissue; cellular imaging; cryostat; density; design; digital; experience; insight; investigator training; laser capture microdissection; neurochemistry; neuropathology; professor; receptor binding

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As is the case for all areas of neuroscience research, the application of modern methods of cellular imaging are greatly accelerating advancements in the field. The center's Imaging Core gives investigators access to state-of-the-art, sophisticated microscopic equipment with advanced capabilities in the visualization, quantification and interpretation of neuronal systems at the cellular level. Dr. Grazyna Rajkowska, (Professor of Psychiatry and Human Behavior; Head of the Quantitative Neuroanatomy Laboratory), serves as Core Director of the CPN's Imaging Core. Dr. Rajkowska is a neuroanatomist with 20 years experience in quantitative image analysis of specific types of neurons and glial cells in human postmortem and animal brain tissues. She has served as Head of the Laboratory of Quantitative Neuroanatomy at UMC for the past 13 years and has published over 100 scientific papers and abstracts in the field of quantitative morphology. Dr. Rajkowska provides oversight of investigator training in the use of core equipment, the maintenance of core equipment, and the supervision of core personnel. In addition to the administrative oversight of the Imaging Core, Dr. Rajkowska instructs Center investigators and other UMC researchers in the general use of the microscopic imaging equipment and further offers highly specialized expertise in the technical parameters relevant to the design, application and interpretation of quantitative morphological and neurochemical studies. These technical parameters include tissue slicing and general histological staining of human postmortem and animal brain tissues, the application of 3-dimensional cell counting techniques to different types of staining procedures, the determination of optimal conditions for immunohistochemistry and guidance in the anatomical localization of the specific brain regions being studied. Dr. Jose Javier Miguel-Hidalgo (Assistant Professor of Psychiatry & Human Behavior, University of Mississippi Medical Center) is a neuroanatomist with 18 years experience in microscopy, histology and histochemical processing of brain samples in a variety of vertebrate species. Dr. Miguel-Hidalgo received formal training in confocal microscopy in 1992 at the University of California at Davis. CPN and UMC investigators often use confocal microscopy for double immunostaining with fluorescent antibodies. Dr. Miguel-Hidalgo also received instruction directly from Arcturus technical field trainers in 2005 and has subsequently trained several researchers at UMC using laser capture microdissection in their research. He further assists Dr. Rajkowska in determining the technical parameters (tissue sectioning, measurement, sampling, etc.) that would produce the best images for each investigator's particular application. The CPN's Imaging Core and UMC currently support a Imaging Facility providing shared use of the following imaging equipment: + Nikon C1 Laser Scanning Confocal Microscope + Arcturus Laser Capture Microdissection System with fluorescence and 60X objective + Kodak Imaging Station + Fuji BAS-5000 Phosphor Image Analysis System + MCID Image Analysis Systems (2) + AIS Image Analysis Workstation + StereoInvestigator and Neurolucida Quantitative Cell Counting System + 3-D Cell Counting System (Williams & Rakic) + Bioquant Cell Counting and Imaging System + Nikon Light Microscope, with stereoinvestigator software + Leica Stereomicroscope + Zeiss Axioskop Microscopes (2) + Axioskop Digital Photomicroscopy Workstation. + Leica Cryostat These sophisticated imaging systems give investigators the ability to estimate, in three-dimensional space, numbers of cells, terminals, or synapses and to analyze the density of receptor binding and the level of specific proteins, giving researchers unprecedented insight into the cellular and molecular neuropathology of mental disorders.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 与神经科学研究的所有领域一样，现代细胞成像方法的应用大大加速了该领域的进步。该中心的成像核心为研究人员提供了最先进的，复杂的显微设备，具有在细胞水平上可视化，定量和解释神经系统的先进能力。 博士Grazyna Rajkowska（精神病学和人类行为教授;定量神经解剖学实验室负责人）担任CPN成像核心的核心主任。Rajkowska博士是一位神经解剖学家，在人类死后和动物脑组织中特定类型的神经元和神经胶质细胞的定量图像分析方面拥有20年的经验。在过去的13年里，她一直担任UMC定量神经解剖学实验室的负责人，并在定量形态学领域发表了100多篇科学论文和摘要。Rajkowska博士负责监督研究者在核心设备使用、核心设备维护和核心人员监督方面的培训。 除了对成像核心进行行政监督外，Rajkowska博士还指导中心研究人员和其他UMC研究人员一般使用显微成像设备，并进一步提供与定量形态学和神经化学研究的设计，应用和解释相关的技术参数方面的高度专业知识。这些技术参数包括人类死后和动物脑组织的组织切片和一般组织学染色，三维细胞计数技术在不同类型的染色程序中的应用，确定免疫组织化学的最佳条件，并指导正在研究的特定脑区域的解剖定位。 博士Jose Javier Miguel-Hidalgo（密西西比大学医学中心精神病学与人类行为助理教授）是一位神经解剖学家，在各种脊椎动物大脑样本的显微镜、组织学和组织化学处理方面拥有18年的经验。Miguel-Hidalgo博士于1992年在位于戴维斯的加州大学接受了共聚焦显微镜的正式培训。CPN和UMC研究人员经常使用共聚焦显微镜进行荧光抗体双重免疫染色。Miguel-Hidalgo博士还在2005年直接接受了大角星技术领域培训师的指导，随后在UMC培训了几名研究人员，在他们的研究中使用激光捕获显微切割。他还协助Rajkowska博士确定技术参数（组织切片、测量、取样等）。这将为每个研究者的特定应用产生最好的图像。 CPN的成像核心和UMC目前支持一个成像设施，提供以下成像设备的共享使用： + 尼康C1激光扫描共聚焦显微镜 + Arcturus激光捕获显微切割系统，带荧光和60倍物镜 + 柯达影像站 + 富士BAS-5000荧光图像分析系统 + MCID图像分析系统（2） + AIS图像分析工作站 + StereoInvestigator和Neurolucida定量细胞计数系统 + 三维细胞计数系统（威廉姆斯& Rakic） + Bioquant细胞计数和成像系统 + 尼康光学显微镜，带立体调查软件 + Leica立体显微镜 + 蔡司Axioskop显微镜（2） + Axioskop数字显微镜工作站。 + Leica低温恒温器 这些复杂的成像系统使研究人员能够在三维空间中估计细胞、终端或突触的数量，并分析受体结合的密度和特定蛋白质的水平，使研究人员对精神障碍的细胞和分子神经病理学有了前所未有的了解。