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中文摘要
翻译
这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金, 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 分子生物学/功能基因组学子模块位于俄克拉荷马医学研究基金会(阵列分析)、细胞生物学和眼科(设备室)和细胞生物学(胚胎冷冻保存室)。设备子模块中设备的使用和维护由Muayyad Al-Ubaidi博士协调。 微阵列设备为俄克拉荷马州的视力科学家提供服务,包括实验设计、RNA质量控制、标记、微阵列打印、杂交、图像分析以及数据的统计和生物信息学分析。 体外和体内RNAi设施提供了在体外和体内进行基因敲除所需的所有必要设备。 核心胚胎冷冻设备被VISION研究人员广泛用于冷冻保存小鼠胚胎,以避免维持小鼠群体所需的成本和工作量,防止灾难(物理和病理),防止遗传漂移,清理病原体系列,并简化向其他机构的线路运输。
英文摘要
This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The molecular biology/functional genomics sub-modules are located in the Oklahoma Medical Research Foundation (array analysis), in the Department of Cell Biology and Ophthalmology (equipment rooms), and in Cell Biology (embryo cryo-preservation room). The use and maintenance of the equipment in the equipment sub-module are coordinated by Muayyad Al-Ubaidi, Ph.D. The Microarray Facility provides services to Oklahoma's vision scientists, including consultation on experimental design, RNA QC, labeling, microarray printing, hybridization, image analysis, and statistical and bioinformatics analyses of data. The in vitro and in vivo RNAi facilities provide all necessary equipment necessary to perform the gene knockdowns, both in vitro and in vivo. The Core Embryonic Freezing Facility is used extensively by vision researchers to cryopreserve mouse embryos to avoid the cost and effort required to maintain colonies of mice, to insure against catastrophe (physical, pathological), to prevent genetic drift, to clean a line of pathogens, and to simplify shipping of lines to other institutions.
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Retinal degeneration caused by alterations in protein tyrosine O-sulfation
Retinal degeneration caused by alterations in protein O-sulfation
Compacted DNA Nanoparticles for Ocular Therapy
  • 批准号:
    10246311
  • 项目类别:
  • 资助金额:
    $54.81万
  • 财政年份:
    2008
  • 负责人:
    MUAYYAD R AL-UBAIDI
  • 依托单位: