MOLECULAR BIOLOGY/FUNCTIONAL GENOMICS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 分子生物学/功能基因组学子模块位于俄克拉荷马州医学研究基金会（阵列分析）、细胞生物学和眼科学系（设备室）和细胞生物学（胚胎冷冻保存室）。设备子模块中设备的使用和维护由Muayyad Al-Ubaidi博士协调。 微阵列设施为俄克拉荷马州的视觉科学家提供服务，包括实验设计咨询、RNA QC、标记、微阵列打印、杂交、图像分析以及数据的统计和生物信息学分析。 体外和体内RNAi设施提供了在体外和体内进行基因敲减所需的所有必要设备。 核心胚胎冷冻设施被视觉研究人员广泛用于冷冻保存小鼠胚胎，以避免维持小鼠群体所需的成本和努力，以防止灾难（物理，病理），防止遗传漂变，清除病原体，并简化将线路运送到其他机构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The molecular biology/functional genomics sub-modules are located in the Oklahoma Medical Research Foundation (array analysis), in the Department of Cell Biology and Ophthalmology (equipment rooms), and in Cell Biology (embryo cryo-preservation room). The use and maintenance of the equipment in the equipment sub-module are coordinated by Muayyad Al-Ubaidi, Ph.D. The Microarray Facility provides services to Oklahoma's vision scientists, including consultation on experimental design, RNA QC, labeling, microarray printing, hybridization, image analysis, and statistical and bioinformatics analyses of data. The in vitro and in vivo RNAi facilities provide all necessary equipment necessary to perform the gene knockdowns, both in vitro and in vivo. The Core Embryonic Freezing Facility is used extensively by vision researchers to cryopreserve mouse embryos to avoid the cost and effort required to maintain colonies of mice, to insure against catastrophe (physical, pathological), to prevent genetic drift, to clean a line of pathogens, and to simplify shipping of lines to other institutions.