CRYSTALLOGRAPHIC STUDIES OF TRANSPORTERS AND RECEPTORS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们有多个完整的膜蛋白转运蛋白和配体门控离子通道受体，包括GltPh天冬氨酸转运蛋白，LeuT亮氨酸转运蛋白和两个真核生物配体门控离子通道，目前没有高分辨率的结构数据的晶体。 我们的目标是进行具体的机制研究GltPh和LeuT和进行MAD（SeMet）实验上的两个配体门控离子通道蛋白，以解决相位问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have crystals of multiple integral membrane protein transporters and ligand-gated ion channel receptors that include the GltPh aspartate transporter, LeuT leucine transporter and two eukaryotic ligand-gated ion channels for which there is currently no high resolution structural data. We aim to carry out specific mechanistics studies on GltPh and LeuT and to perform MAD (SeMet) experiments on the two ligand-gated ion channel proteins in order to solve the phase problem.