STRUCTURAL ANALYSIS OF MEMBRANE PROTEINS INVOLVED IN BACTERIAL PATHOGENICITY

涉及细菌致病性的膜蛋白的结构分析

• 批准号: 7722009
• 负责人: NATALIE CJ STRYNADKA
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722009
• 关键词: Cells; Computer Retrieval of Information on Scientific Projects Database; Data; Funding; Grant; Institution; Membrane; Membrane Proteins; Methionine; Pathogenicity; Research; Research Personnel; Resources; Source; Synchrotrons; System; United States National Institutes of Health; Work; improved; novel; pathogen; protein expression

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This work will involve the novel structural analysis of previously uncharacterized membrane components of a secretion system unique to bacterial pathogens. The crystals generally are very small with large cells and improve significantly with synchrotron sources. We will collect native and seleno-methionine data, as well as potentially heavy atom data (some of the projects have very poor protein expression being membrane proteins and so seleno-met is not possible).

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项工作将涉及对细菌病原体特有的分泌系统的先前未表征的膜成分的新颖结构分析。晶体通常很小，晶胞很大，使用同步加速器可以显著提高晶体质量。我们将收集天然和硒蛋氨酸的数据，以及潜在的重原子数据(其中一些项目的膜蛋白的蛋白质表达非常差，因此硒甲硫氨酸是不可能的)。