STRUCTURAL ANALYSIS OF MEMBRANE PROTEINS INVOLVED IN BACTERIAL PATHOGENICITY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 这项工作将涉及新的结构分析以前未知的膜成分的分泌系统独特的细菌病原体。晶体通常非常小，具有大的细胞，并且使用同步加速器源显着改善。我们将收集天然和硒蛋氨酸数据，以及潜在的重原子数据（一些项目的蛋白质表达非常差，是膜蛋白，因此硒蛋氨酸是不可能的）。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This work will involve the novel structural analysis of previously uncharacterized membrane components of a secretion system unique to bacterial pathogens. The crystals generally are very small with large cells and improve significantly with synchrotron sources. We will collect native and seleno-methionine data, as well as potentially heavy atom data (some of the projects have very poor protein expression being membrane proteins and so seleno-met is not possible).