THREE-DIMENSIONAL STRUCTURE AND FUNCTION OF THE MAMMALIAN KINETOCHORE

哺乳动物动粒的三维结构和功能

• 批准号: 7721694
• 负责人: BRUCE F MCEWEN
• 金额: $ 3.34万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721694
• 关键词: Anaphase; Cell Cycle Checkpoint; Chromosomes; Complex; Computer Retrieval of Information on Scientific Projects Database; Electron Microscopy; Funding; Grant; Institution; Kinetochores; Microtubules; Mitosis; Mitotic spindle; Molecular; Play; Process; Research; Research Personnel; Resources; Source; United States National Institutes of Health; reconstruction; three dimensional structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The mammalian kinetochore is a proteineous complex that performs four vital functions during mitosis: 1) attaching chromosomes to the mitotic spindle; 2) controlling the dynamics of kinetochore microtubules; 3) generating force for chromosome alignment; and 4) generating a cell cycle checkpoint that delays anaphase onset until all chromosomes are attached to the mitotic spindle and aligned at the spindle equator. Intricate interactions between kinetochores and microtubules (MTs) are essential for all of these vital processes. Despite rapid progress in identifying molecular components of the kinetochore, understanding of the underlying mechanisms of kinetochore function and its interactions with microtubules is just beginning to unfold. Results from tomographic reconstructions and serial section electron microscopy are playing a significant part in deducing the function of key molecular components such as the Hec1 (Ndc80) complex.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 哺乳动物动粒是一种蛋白质复合体，在有丝分裂期间执行四种重要功能：1）将染色体附着到有丝分裂纺锤体; 2）控制动粒微管的动力学; 3）产生染色体对齐的力;以及4）产生细胞周期检查点，其延迟后期开始，直到所有染色体附着到有丝分裂纺锤体并在纺锤体赤道对齐。动粒和微管（MT）之间复杂的相互作用是所有这些重要过程所必需的。 尽管在确定动粒的分子组成方面取得了快速进展，但对动粒功能及其与微管相互作用的潜在机制的理解才刚刚开始。 断层重建和连续切片电子显微镜的结果在推断关键分子组分如Hec 1（Ndc 80）复合物的功能方面发挥着重要作用。