PROTEIN STRUCTURE DETERMINATION USING CROSS-LINKING, MASS SPECTROMETRY, MODELING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们开发了一种方法来确定蛋白质的低分辨结构，从质谱学和化学交联法得出的距离约束。尽管X射线结晶学和核磁共振产生了高分辨率的结构，但它们有局限性：前者需要衍射晶体，后者需要相对较低分子量的蛋白质。我们的新方法没有这些限制。 我们已经使用MidasPlus和Chimera帮助生成同源模型结构(使用swappaa命令)并显示此类结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We developed a method to determine low resolution structures of proteins from distance constraints derived from mass spectrometry and chemical cross-linking. Although x-ray crystallography and NMR generate high resolution structures, they have limitations: the demand for diffracting crystals, in the first case, and relatively low molecular weight proteins, in the latter. Our new methodology does not have these limitations. We have used MidasPlus and Chimera to help generate homology-modelled structures (using the command swappaa) and to display such structures.