PROTEIN STRUCTURE DETERMINATION USING CROSS-LINKING, MASS SPECTROMETRY, MODELING

使用交联、质谱、建模确定蛋白质结构

• 批准号: 7367744
• 负责人: CONNIE M OSHIRO
• 金额: $ 0.77万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7367744
• 关键词: PROTEIN; STRUCTURE; DETERMINATION; USING; CROSS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are currently developing a method to determine low resolution structures of proteins from distance constraints derived from mass spectrometry and chemical cross-linking. Although x-ray crystallography and NMR generate high resolution structures, they have limitations: the demand for diffracting crystals, in the first case, and relatively low molecular weight proteins, in the latter. This new methodology would not have this limitation. Thus, we would be able to consider, eventually, many more targets. We have used MidasPlus and Chimera to help generate homology-modelled structures (using the command swappaa) and to display such structures.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们目前正在开发一种方法来确定低分辨率的蛋白质结构的距离限制来自质谱和化学交联。虽然X射线晶体学和NMR产生高分辨率的结构，但它们有局限性：在第一种情况下需要衍射晶体，而在后者中需要相对低分子量的蛋白质。这种新的方法将不会有这种限制。因此，我们最终将能够考虑更多的目标。我们使用MidasPlus和Chimera来帮助生成同源建模结构（使用命令swappaa）并显示这些结构。