PROTEIN STRUCTURE DETERMINATION USING CROSS-LINKING, MASS SPECTROMETRY, MODELING

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们目前正在开发一种方法，根据质谱学和化学交联法得出的距离限制来确定蛋白质的低分辨率结构。尽管X射线结晶学和核磁共振产生了高分辨率的结构，但它们有局限性：前者需要衍射晶体，后者需要相对较低分子量的蛋白质。这种新的方法将不会有这种限制。因此，我们最终将能够考虑更多的目标。我们已经使用MidasPlus和Chimera帮助生成同源模型结构(使用swappaa命令)并显示此类结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are currently developing a method to determine low resolution structures of proteins from distance constraints derived from mass spectrometry and chemical cross-linking. Although x-ray crystallography and NMR generate high resolution structures, they have limitations: the demand for diffracting crystals, in the first case, and relatively low molecular weight proteins, in the latter. This new methodology would not have this limitation. Thus, we would be able to consider, eventually, many more targets. We have used MidasPlus and Chimera to help generate homology-modelled structures (using the command swappaa) and to display such structures.