STRUCTURE DETERMINATION OF PROTEINS INVOLVED IN DNA REPLICATIOM

DNA 复制涉及的蛋白质的结构测定

• 批准号: 7722282
• 负责人: David Jeruzalmi
• 金额: $ 0.11万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722282
• 关键词: Complex; Computer Retrieval of Information on Scientific Projects Database; DNA biosynthesis; Funding; Grant; Institution; MCM Protein; Protein Overexpression; Protein S; Proteins; Purpose; Research; Research Personnel; Resources; Roentgen Rays; Saccharomyces cerevisiae; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure; United States National Institutes of Health; origin recognition complex; reconstitution; three dimensional structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. An ongoing project aimed at confirming, with MALDI-MS, the identification of various proteins involved in DNA replication. These proteins are being overexpressed and reconstituted for the purpose of X-Ray crystallographic determination of their three-dimensional structures. MCM proteins from S. cerevisiae and B. subtilus, and ORC proteins from S. cerevisiae are among the protein complexes under study.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 一个正在进行的项目，旨在确认，与MALDI-MS，识别各种蛋白质参与DNA复制。 这些蛋白质被过度表达和重组，用于X射线晶体学测定其三维结构。 S.酿酒酵母和B. subtilus和S.酿酒酵母是正在研究的蛋白质复合物之一。