STRUCTURE DETERMINATION OF PROTEINS INVOLVED IN DNA REPLICATIOM

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 一个正在进行的项目，旨在用MALDI-MS确认与DNA复制有关的各种蛋白质的鉴定。这些蛋白质被过度表达和重组，用于X射线结晶学测定其三维结构。来自酿酒酵母和枯草芽孢杆菌的MCM蛋白，以及来自酿酒酵母的ORC蛋白是正在研究的蛋白质复合体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. An ongoing project aimed at confirming, with MALDI-MS, the identification of various proteins involved in DNA replication. These proteins are being overexpressed and reconstituted for the purpose of X-Ray crystallographic determination of their three-dimensional structures. MCM proteins from S. cerevisiae and B. subtilus, and ORC proteins from S. cerevisiae are among the protein complexes under study.