SAXS STUDIES OF PROTEIN COMPLEXES INVOLVED IN DNA REPAIR AND REPLICATION

参与 DNA 修复和复制的蛋白质复合物的 SAXS 研究

• 批准号: 8168657
• 负责人: David Jeruzalmi
• 金额: $ 1.08万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168657
• 关键词: Address; Bacterial DNA; Biological Process; Cell Cycle; Cell Proliferation; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Repair Pathway; DNA biosynthesis; DNA lesion; Ensure; Funding; Genetic; Genome; Genomics; Goals; Grant; Institution; Life; Maintenance; Modeling; Molecular Weight; Nucleoproteins; Nucleotide Excision Repair; Organism; Pathway interactions; Personal Satisfaction; Phase; Process; Research; Research Personnel; Resources; Roentgen Rays; Shapes; Source; Structure; United States National Institutes of Health; Work; in vivo; protein complex; repaired; senescence; tumorigenesis

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Genomic integrity is essential for the well-being and survival of organisms. Cells spend tremendous energy to protect the information held within the DNA. DNA replication is highly regulated and coordinated to ensure that the entire genome is replicated once and only once per cell division cycle. Further, various DNA lesions are recognized and processed by different DNA repair pathways. The goal of this work is to use small-angle X-ray scattering to address several long-standing questions about protein complexes that function in the nucleotide excision repair pathway and nucleoprotein assemblies that are formed during the initiation phase of bacterial DNA replication. We will obtain information on the size, shape, and molecular weight of these complexes. The information will be used to formulate models of how they function in vivo. Understanding the structure and function of these assemblies is important as the maintenance of genetic information is a fundamental process shared by all kingdoms of life, and will impact how we view DNA replication and repair, cell proliferation, and other biological processes such as senescence and tumorigenesis.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 基因组的完整性对生物体的健康和生存至关重要。细胞花费巨大的能量来保护DNA中的信息。 DNA复制受到高度调节和协调，以确保整个基因组在每个细胞分裂周期复制一次且仅复制一次。 此外，各种DNA损伤通过不同的DNA修复途径识别和处理。这项工作的目标是使用小角X射线散射来解决几个长期存在的问题，蛋白质复合物的功能，在核苷酸切除修复途径和核蛋白组装，在细菌DNA复制的起始阶段形成。 我们将获得有关这些复合物的大小、形状和分子量的信息。 这些信息将用于制定它们如何在体内发挥作用的模型。了解这些组件的结构和功能是重要的，因为遗传信息的维护是所有生命王国共享的基本过程，并将影响我们如何看待DNA复制和修复，细胞增殖以及其他生物过程，如衰老和肿瘤发生。