SAXS STUDIES OF PROTEIN COMPLEXES INVOLVED IN DNA REPAIR AND REPLICATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 基因组的完整性对生物体的福祉和生存至关重要。细胞花费巨大的能量来保护DNA中保存的信息。DNA复制受到高度调控和协调，以确保整个基因组复制一次，并且在每个细胞分裂周期只复制一次。此外，不同的DNA损伤由不同的DNA修复途径识别和处理。这项工作的目标是利用小角X射线散射来解决几个长期存在的问题，这些问题是关于在核苷酸切除修复途径中发挥功能的蛋白质复合体和在细菌DNA复制的起始阶段形成的核蛋白组件。我们将获得有关这些络合物的大小、形状和分子量的信息。这些信息将被用来建立它们在体内如何发挥作用的模型。了解这些组件的结构和功能是重要的，因为维持遗传信息是所有生命王国共享的基本过程，并将影响我们如何看待DNA复制和修复、细胞增殖以及其他生物过程，如衰老和肿瘤发生。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Genomic integrity is essential for the well-being and survival of organisms. Cells spend tremendous energy to protect the information held within the DNA. DNA replication is highly regulated and coordinated to ensure that the entire genome is replicated once and only once per cell division cycle. Further, various DNA lesions are recognized and processed by different DNA repair pathways. The goal of this work is to use small-angle X-ray scattering to address several long-standing questions about protein complexes that function in the nucleotide excision repair pathway and nucleoprotein assemblies that are formed during the initiation phase of bacterial DNA replication. We will obtain information on the size, shape, and molecular weight of these complexes. The information will be used to formulate models of how they function in vivo. Understanding the structure and function of these assemblies is important as the maintenance of genetic information is a fundamental process shared by all kingdoms of life, and will impact how we view DNA replication and repair, cell proliferation, and other biological processes such as senescence and tumorigenesis.