ANALYSIS OF THE MICROTUBULE-ASSOCIATED PROTEOME DURING NEURONAL MORPHOGENESIS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在神经细胞发育过程中，有丝分裂后的神经元从表面上未极化的细胞转变为高度极化的细胞。这些高度极化的神经元含有被称为神经突的长细胞突起，其充满微管（MT）细胞骨架，并且稍后将成熟为轴突或树突。研究表明，神经元发育过程中的形态学变化依赖于MT细胞骨架的组织结构。虽然许多MT相关蛋白在神经元形态发生过程中起着重要作用，但缺乏对MT细胞骨架的全面蛋白质组学分析。为了更好地了解控制神经突形成的机制，我们建议在此过程之前和过程中对MT相关蛋白进行基于MS的蛋白质组学分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During nerve cell development, postmitotic neurons transform from seemingly unpolarized cells into highly polarized ones. These highly polarized neurons contain long cellular processes termed neurites that are filled with the microtubule (MT) cytoskeleton, and will later mature into axons or dendrites. It has been shown that the morphological change during neuronal development depends on the organization of the MT cytoskeleton. Although many of MT-associated proteins are known to play important roles during neuronal morphogenesis, a comprehensive proteomic analysis focusing on the MT cytoskeleton during this process is lacking. To better understand the mechanisms controlling neurite formation, we propose to perform MS-based proteomic analysis on MT-associated proteins before and during this process.