GENETIC AND BIOCHEMICAL STUDIES OF THE KSHV LANA GENE: AIDS OI

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 卡波西肉瘤相关疱疹病毒(KSHV)或人类疱疹病毒8型(HHV8)是一种与卡波西肉瘤、原发渗出性淋巴瘤和多中心Castleman病相关的伽玛2型疱疹病毒。KSHV主要是潜伏的，双链DNA基因组保持为环状的、染色体外的Episome(质粒)，从中只表达一部分病毒基因，包括潜伏相关核抗原(LANA)。在有丝分裂过程中，LANA既需要复制病毒基因组，也需要将病毒基因组分配给后代核；LANA通过将KSHV DNA拴在有丝分裂染色体上来分割上体。LANA的N端通过核心组蛋白附着在染色体上。这项研究的目的是鉴定LANA同源物可能需要的相互作用的细胞蛋白，这些蛋白可能是来自其他伽玛-2疱疹病毒的LANA同源物附着到有丝分裂染色体上所必需的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Kaposi's Sarcoma-associated herpesvirus (KSHV), or human herpesvirus 8 (HHV8), is a gamma-2-herpesvirus linked with Kaposi's Sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. KSHV is predominantly latent, with the double-stranded DNA genome maintained as a circular, extrachromosomal episome (plasmid) from which only a subset of viral genes is expressed, including latency-associated nuclear antigen (LANA). LANA is required to both replicate and partition viral genomes to progeny nuclei during mitosis; LANA partitions episomes by tethering KSHV DNA to mitotic chromosome. The N-terminus of LANA attaches to chromosomes through core histones. This research aims to identify the interacting cellular proteins that may be required for LANA homologs from other gamma-2-herpesviruses to attach to mitotic chromosomes.