GENETIC AND BIOCHEMICAL STUDIES OF THE KSHV LANA GENE: AIDS OI

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，但不一定是研究者所在的机构。 卡波西肉瘤相关疱疹病毒（KSHV）或人类疱疹病毒8（HHV 8）是一种与卡波西肉瘤，原发性渗出性淋巴瘤和多中心Castleman病相关的γ-2-疱疹病毒。KSHV主要是潜伏性的，双链DNA基因组保持为环状的染色体外附加体（质粒），仅表达一部分病毒基因，包括潜伏相关核抗原（拉娜）。在有丝分裂期间，需要拉娜复制病毒基因组并将其分配到子代细胞核;拉娜通过将KSHV DNA拴系到有丝分裂染色体来分配附加体。拉娜的N-末端通过核心组蛋白附着于染色体。本研究的目的是确定相互作用的细胞蛋白质，可能需要从其他γ-2-疱疹病毒的拉娜同源物附着到有丝分裂染色体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Kaposi's Sarcoma-associated herpesvirus (KSHV), or human herpesvirus 8 (HHV8), is a gamma-2-herpesvirus linked with Kaposi's Sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. KSHV is predominantly latent, with the double-stranded DNA genome maintained as a circular, extrachromosomal episome (plasmid) from which only a subset of viral genes is expressed, including latency-associated nuclear antigen (LANA). LANA is required to both replicate and partition viral genomes to progeny nuclei during mitosis; LANA partitions episomes by tethering KSHV DNA to mitotic chromosome. The N-terminus of LANA attaches to chromosomes through core histones. This research aims to identify the interacting cellular proteins that may be required for LANA homologs from other gamma-2-herpesviruses to attach to mitotic chromosomes.