) DETECTION AND QUANTIFICATION OF THE ANTIOXIDANT PROFILE IN THE HUMAN BRAIN

) 人脑中抗氧化成分的检测和定量

• 批准号: 7721373
• 负责人: Melissa J Terpstra
• 金额: $ 3.57万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721373
• 关键词: Affect; Alzheimer' s Disease; Antioxidants; Brain; Computer Retrieval of Information on Scientific Projects Database; Condition; Detection; Docosahexaenoic Acids; Funding; Glutathione; Glutathione Disulfide; Grant; Human; In Vivo NMR Spectroscopy; Institution; Link; Measures; Methods; Neurodegenerative Disorders; Non-Invasive Cancer Detection; Oxidative Stress; Parkinson Disease; Physiologic pulse; Physiological; Pulse taking; Research; Research Personnel; Resolution; Resources; Source; United States National Institutes of Health; ascorbate

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Oxidative stress is linked with neurodegenerative diseases, such as Parkinson's or Alzheimer's, and brain antioxidants (glutathione, GSH; ascorbate, Asc) have a major protective function. Non-invasive detection and quantification of these major antioxidants in human brain is possible exclusively using in vivo NMR spectroscopy. To increase the reliability of quantification, a double editing pulse sequence was developed for simultaneous detection of both antioxidants (Terpstra et al. 2006b). Detection of antioxidant levels in the human brain is unique for the high field due to increased spectra resolution. In addition, spectral parameters of oxidized forms of ascorbate (DHA) and glutathione (GSSG) were measured at physiological conditions to estimate their contribution in GSH and Asc quantification. It was demonstrated that quantification of reduced forms of antioxidants was not affected by their oxidized forms, because of spectral differences between Asc and DHA and between GSH and GSSG and the spectral selectivity of the editing method.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 氧化应激与神经退行性疾病（例如帕金森氏症或阿尔茨海默氏病）以及脑抗氧化剂（谷胱甘肽，GSH；抗坏血酸，ASC）有关。可能仅使用体内NMR光谱法仅使用侵入性检测和对人脑中这些主要抗氧化剂的定量。为了提高定量的可靠性，开发了双重编辑脉冲序列，以同时检测两种抗氧化剂（Terpstra等，2006b）。由于光谱分辨率增加，人脑中抗氧化剂水平的检测对于高场是独一无二的。此外，在生理条件下测量了抗坏血酸（DHA）和谷胱甘肽（GSSG）的氧化形式的光谱参数，以估计其在GSH和ASC定量中的贡献。已经证明，由于ASC和DHA之间以及GSH和GSSG之间的频谱差异以及编辑方法的光谱选择性，对还原形式的抗氧化剂形式的定量不受其氧化形式的影响。