IN VIVO APOPTOSIS & MITOCHONDRIA

体内细胞凋亡

• 批准号: 7722313
• 负责人: GUY A PERKINS
• 金额: $ 0.32万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722313
• 关键词: 3-Dimensional; Aerobic; Apoptosis; Architecture; Biochemistry; Cell Respiration; Comparative Study; Computer Retrieval of Information on Scientific Projects Database; Crista ampullaris; Dimensions; Electron Microscope; Funding; Goals; Grant; Institution; Link; Membrane; Mitochondria; Mitochondrial Crista; Mus; Numbers; Oxygen Consumption; Photoreceptors; Primates; Reporting; Research; Research Personnel; Resolution; Resources; Retina; Retinal Cone; Site; Source; Structure; Techniques; United States National Institutes of Health; Vertebrate Photoreceptors; comparative; enzyme activity; in vivo; retinal rods; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The basic structure, biochemistry and function of mammalian retinal rod and cone photoreceptors are well described. However, there are relatively few reports on the ultrastructural details of rod and cone mitochondria and even fewer on their comparative aspects. The 3-D technique with the highest resolution available for studying mitochondria is electron microscope tomography. Comparative studies of structure related to function offer a promising means of understanding the significance of differences in cytoarchitecture. Mitochondrial crista structure is linked tightly to mitochondrial function. Non-foveal cone photoreceptors of primates contain considerably more inner segment mitochondria and have higher oxidative enzyme activity than do rods. In addition, it is suggested that light-adapted cones have a higher aerobic ATP demand than rods. Therefore, we investigated the oxidative metabolism and three-dimensional membrane architecture of mouse rod and cone inner segment mitochondria. Our goals were to determine, in light-adapted mouse retinas, the: 1) number of mitochondria and oxidative enzyme activity in rod inner segment (RIS) and cone inner segment (CIS), 2) photoreceptor oxygen consumption (QOPR) and compare it to that in dark-adapted photoreceptors, 3) dimensions and connectivity of RIS and CIS mitochondrial cristae and contact sites, and 4) structural motifs of RIS and CIS cristae.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 哺乳动物视网膜视杆细胞和视锥细胞的基本结构、生物化学和功能已被很好地描述。然而，有相对较少的报道，杆和锥线粒体的超微结构的细节，甚至更少的比较方面。可用于研究线粒体的分辨率最高的3D技术是电子显微镜断层扫描。结构与功能相关的比较研究为理解细胞结构差异的意义提供了一种有前途的方法。线粒体嵴结构与线粒体功能密切相关。灵长类动物的非中央凹视锥光感受器含有相当多的内节线粒体，并具有比视杆细胞更高的氧化酶活性。此外，有人建议，光适应锥有更高的有氧ATP需求比杆。因此，我们研究了小鼠视杆细胞和视锥细胞内节线粒体的氧化代谢和三维膜结构。我们的目标是确定，在光适应的小鼠视网膜中：1）在杆内段（RIS）和锥内段（CIS）中的线粒体数量和氧化酶活性，2）光感受器耗氧量（QOPR）并将其与暗适应的光感受器进行比较，3）RIS和CIS线粒体嵴和接触位点的尺寸和连接性，以及4）RIS和CIS嵴的结构基序。