INSECT FLIGHT MUSCLE HOT, COLD, PULLED AND PULLING
昆虫飞行肌肉热、冷、拉和拉
基本信息
- 批准号:7722742
- 负责人:
- 金额:$ 3.16万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-04-01 至 2008-12-31
- 项目状态:已结题
- 来源:
- 关键词:CalciumComputer Retrieval of Information on Scientific Projects DatabaseElasticityFiberFilamentFreezingFundingGrantImageInsectaInstitutionIsometric ExerciseLengthMicrofilamentsMicrotomyMotorMuscleMyosin ATPasePatternProtocols documentationRanaResearchResearch PersonnelResourcesRoentgen RaysSarcomeresSchemeSkeletal systemSourceStretchingStriated MusclesStructureTemperatureThick FilamentTimeUnited States National Institutes of HealthWorkX ray diffraction analysisX-Ray Diffractionmembernovelprogramsresearch studyresponsetomography
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Our program combines & compares X-ray diffraction (glycerinated native fibers) and thin-section EM tomography (fibers quick-frozen & freeze-substituted) to characterize structure, arrangement and dynamic choreography of myosin crossbridges (motor molecules of muscle) in a waterbug insect flight muscle (IFM) of unexcelled crystalline regularity. All X-ray experiments proposed will, whenever revealing, be matched by parallel EM experiments on fibers quick-frozen during the same state or maneuver, to directly image the structural changes.) X-ray patterns from IFM differ in important ways from those of other striated muscles (frog skeletal) studied by XRD, owing to differences in filament lattice packing, in thick filament helical geometry, and a full-overlap sarcomere scheme limited to ~3% length changes. X-ray reflections (to ~5 nm) attributable to myosin filaments, actin filaments, and both jointly, are as well worked out as in frog muscle, but not yet so well studied in dynamic (contraction) states. The present proposal is to continue our novel recent work on crossbridge braking actions, and to begin time-resolved perturbations of active IFM planned with members of Vincenzo Lombardi's lab. This present work will continue analysis to 4 nm and higher of muscle dynamic structural responses to relaxed, rigor, and active states, to temperatures 1 to 40¿ C (IFM requires preflight warm up to ~40¿C to enable flight), to rapid stretches that elicit either stretch-activation or crossbridge braking (depending on muscle state), to isometric calcium-activation versus cyclic stretch-activation, and to stretch/release protocols that probe filament elasticity and load-dependent crossbridge distortion.
这个子项目是许多研究子项目中的一个
由NIH/NCRR资助的中心赠款提供的资源。子项目和
研究者(PI)可能从另一个NIH来源获得了主要资金,
因此可以在其他CRISP条目中表示。所列机构为
研究中心,而研究中心不一定是研究者所在的机构。
我们的程序结合和比较X射线衍射(甘油化的天然纤维)和薄切片EM断层扫描(纤维速冻和冷冻取代)来表征肌球蛋白横桥(肌肉的马达分子)的结构,排列和动态编排在一个水蝽昆虫飞行肌肉(IFM)的无与伦比的结晶规律。 所有提出的X射线实验,无论何时揭示,都将与在相同状态或动作期间快速冷冻的纤维上的平行EM实验相匹配,以直接成像结构变化。 来自IFM的X射线图案与通过XRD研究的其他横纹肌(青蛙骨骼肌)的X射线图案在重要方面不同,这是由于细丝晶格堆积、粗细丝螺旋几何形状和限制于~3%长度变化的全重叠肌节方案的差异。 肌球蛋白丝、肌动蛋白丝以及两者共同产生的X射线反射(~5 nm)与青蛙肌肉中的一样,但在动态(收缩)状态下还没有得到很好的研究。目前的建议是继续我们的新的最近的工作对跨桥制动行动,并开始时间分辨扰动的积极IFM计划与成员的Lombardi的实验室。 目前的工作将继续分析4纳米和更高的肌肉动态结构响应放松,僵硬,和活动状态,温度1至40摄氏度(IFM要求飞行前预热至~40 º C使飞行),快速拉伸,引起拉伸激活或过桥制动(取决于肌肉状态),等长钙激活与循环拉伸激活,以及探测细丝弹性和负载依赖性横桥变形的拉伸/释放方案。
项目成果
期刊论文数量(0)
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MICHAEL KAY REEDY其他文献
MICHAEL KAY REEDY的其他文献
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{{ truncateString('MICHAEL KAY REEDY', 18)}}的其他基金
Structural Dynamics of Stretch Activation in Muscle
肌肉拉伸激活的结构动力学
- 批准号:
8444214 - 财政年份:2012
- 资助金额:
$ 3.16万 - 项目类别:
Structural Dynamics of Stretch Activation in Muscle
肌肉拉伸激活的结构动力学
- 批准号:
8545671 - 财政年份:2012
- 资助金额:
$ 3.16万 - 项目类别:
INSECT FLIGHT MUSCLE: STRETCH-ACTIVATION & FORCE GENERATION
昆虫飞行肌肉:伸展激活
- 批准号:
8361283 - 财政年份:2011
- 资助金额:
$ 3.16万 - 项目类别:
INSECT FLIGHT MUSCLE HOT, COLD, PULLED AND PULLING
昆虫飞行肌肉热、冷、拉和拉
- 批准号:
8168609 - 财政年份:2010
- 资助金额:
$ 3.16万 - 项目类别:
INSECT FLIGHT MUSCLE HOT, COLD, PULLED AND PULLING
昆虫飞行肌肉热、冷、拉和拉
- 批准号:
7954891 - 财政年份:2009
- 资助金额:
$ 3.16万 - 项目类别:
INSECT FLIGHT MUSCLE HOT, COLD, PULLED AND PULLING
昆虫飞行肌肉热、冷、拉和拉
- 批准号:
7601741 - 财政年份:2007
- 资助金额:
$ 3.16万 - 项目类别:
INSECT FLIGHT MUSCLE HOT, COLD, PULLED AND PULLING
昆虫飞行肌肉热、冷、拉和拉
- 批准号:
7369135 - 财政年份:2006
- 资助金额:
$ 3.16万 - 项目类别:
INSECT FLIGHT MUSCLE HOT, COLD, PULLED AND PULLING
昆虫飞行肌肉热、冷、拉和拉
- 批准号:
7182108 - 财政年份:2005
- 资助金额:
$ 3.16万 - 项目类别:
INSECT FLIGHT MUSCLE HOT, COLD, PULLED AND PULLING
昆虫飞行肌肉热、冷、拉和拉
- 批准号:
6975539 - 财政年份:2004
- 资助金额:
$ 3.16万 - 项目类别: