CARDIAC PRESERVATION PRIOR TO TRANSPLANTATION

移植前的心脏保护

• 批准号: 7724106
• 负责人: MICHAEL E JESSEN
• 金额: $ 1.05万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7724106
• 关键词: Animal Model; Biological Preservation; Cardiac; Computer Retrieval of Information on Scientific Projects Database; Condition; Cryopreservation; Funding; Grant; Heart; Heart Diseases; Heart Transplantation; Hour; Implant; Institution; Life; Magic; Magnetic Resonance Spectroscopy; Metabolic; Metabolism; Myocardium; Organ; Perfusion; Physiological reperfusion; Reperfusion Therapy; Research; Research Personnel; Resources; Solutions; Source; Staging; Standards of Weights and Measures; Techniques; Testing; Time; Tissues; Transplantation; United States National Institutes of Health; clinical application; clinically relevant; heart preservation; success; time interval

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Heart transplantation has become a life-saving treatment for end-stage cardiac disease. Its application is limited by the availability of donor hearts and the brief ischemic tolerance of preserved myocardium. Standard cold storage limits preservation intervals to 4 - 6 hours. Recent advances suggest perfusion preservation with oxygenated storage solution may extend the storage interval and permit utilization of marginal donor hearts. An inherent limitation of this technique when applied to long-term storage or use of suboptimal hearts is the difficulty in predicting post-transplant reperfusion function prior to implanting the organ. We previously utilized 1H magnetic resonance spectroscopy (MRS) of cardiac extracts to determine important metabolic differences between hearts preserved under static and perfused conditions. The tissue manipulation and time required to perform this analysis limit its clinical application. Magic angle spinning (MAS) MRS has been used to evaluate metabolism in intact tissue. We hypothesized that 1H MAS MRS could be used to rapidly determine the metabolic status of preserved myocardium. If successful, this would offer the potential to allow assessment of cardiac preservation success in a clinically relevant time interval. To test this possibility, we studied differences between standard static storage and perfusion storage in a large animal model of cardiac transplantation." metabolism.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 心脏移植已成为终末期心脏病的一种挽救生命的治疗方法。 其应用受到供体心脏可获得性和保存心肌短暂缺血耐受性的限制。 标准冷藏限制保存间隔为4 - 6小时。 最近的研究表明，充氧保存液灌注保存可以延长保存时间，并允许边缘供体心脏的利用。 当应用于长期储存或使用次优心脏时，该技术的固有局限性是难以在植入器官之前预测移植后再灌注功能。 我们以前利用心脏提取物的1H磁共振波谱（MRS）来确定在静态和灌注条件下保存的心脏之间的重要代谢差异。 进行该分析所需的组织操作和时间限制了其临床应用。 魔角旋转（MAS）MRS已被用于评估完整组织中的代谢。 我们推测，1H MAS MRS可用于快速确定保存心肌的代谢状态。 如果成功，这将提供在临床相关时间间隔内评估心脏保存成功的可能性。 为了测试这种可能性，我们在一个大型动物心脏移植模型中研究了标准静态储存和灌注储存之间的差异。" 新陈代谢.