CITRIC ACID CYCLE METABOLISM DURING CARDIAC SURGERY

心脏手术期间的柠檬酸循环代谢

• 批准号: 7357885
• 负责人: MICHAEL E JESSEN
• 金额: $ 1.17万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357885
• 关键词: Krebs' cycle; erythrocytes; heart; heart arrest; metabolism; potassium; solutions; surgery

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a series of studies which quantify substrate utilization in pathways designed to replete citric acid cycle intermediates lost during myocardial ischemia under conditions encountered during routine cardiac surgery. The experiments are conducted using an isolated perfused rat heart preparation and involve the use of labeled substrates and NMR spectroscopy. Oxidation of multiple citric acid substrates and anaplerosis are studied under steady-state as well as during and after ischemia in the setting of various cardioplegic solutions. Specifically, the metabolic effects of cardioplegia will be studied, first in control hearts and then in hearts administered 1) warm continuous potassium cardioplegia, 2) an intracellular based cardioplegic solution, 3) low sodium and low potassium cardioplegia, 4) potassium cardioplegia with tetrodotoxin added to achieve polarized arrest, 5) the potassium channel opener pinacidil to achieve hyperpolarized arrest, and 6) washed and packed porcine erythrocytes to evaluate the effects of red blood cells on cellular metaboli

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。这是一系列研究，量化了在常规心脏手术条件下心肌缺血期间柠檬酸循环中间体丢失的途径中的底物利用率。实验使用离体灌注大鼠心脏制备进行，并涉及使用标记底物和NMR光谱。在稳态下以及在各种心脏停搏液的设置中的缺血期间和之后，研究了多种柠檬酸底物的氧化和回补。具体地，将首先在对照心脏中，然后在施用1）温热的连续钾心脏停搏液，2）基于细胞内的心脏停搏液，3）低钠和低钾心脏停搏液，4）添加有河豚毒素的钾心脏停搏液以实现极化停止，5）钾通道开放剂吡那地尔以实现超极化停止，（6）猪红细胞经洗涤、包装后，评价红细胞对细胞代谢的影响。