MEMBRANE MACRO-DOMAINS CHARACTERIZATION W/BACTERIAL LIPOPOLYSACHARIDE

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 我已经使用LFD的装置通过双光子显微镜研究了具有和不具有细菌脂多糖（LPS）的不同膜组合物的Laurdan GP特性。 为了研究LPS与膜的相互作用，我使用DPPC：DOPC（1：1）GUV和LPS-FITC，并且我跟踪膜中的FITC荧光。 为了研究和表征LPS存在下膜中的大结构域，我生长了纯DMPC、DPPC：DOPC（1：1）和DPPC：DOPC：LPS的GUV，并分析了Laurdan的GP性质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. I've used the instalations of the LFD to study the Laurdan GP properties of different membrane composition with and without bacterial lipopolysaccharide (LPS), by two photon microscopy. To study the interaction of LPS with membranes I use DPPC:DOPC (1:1) GUVs and LPS-FITC and I follow the FITC fluorescence in membranes. To study and characterize macrodomains in membranes in presence of LPS I grow up GUVs of DMPC pure, DPPC:DOPC (1:1) and DPPC:DOPC:LPS and analize the GP properties of Laurdan.