MEMBRANE MACRO-DOMAINS CHARACTERIZATION W/BACTERIAL LIPOPOLYSACHARIDE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我利用LFD的装置，用双光子显微镜研究了不同组成的Laurdan GP在细菌脂多糖(LPS)存在和不存在的情况下的性质。 为了研究脂多糖与膜的相互作用，我使用了DPPC：DOPC(1：1)GUS和LPS-FITC，并跟踪了膜上的FITC荧光。 为了研究和表征脂多糖存在下膜中的大分子结构域，我生长了DMPC纯、DPPC：DOPC(1：1)和DPPC：DOPC：LPS的GUV，并分析了劳丹的糖蛋白性质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. I've used the instalations of the LFD to study the Laurdan GP properties of different membrane composition with and without bacterial lipopolysaccharide (LPS), by two photon microscopy. To study the interaction of LPS with membranes I use DPPC:DOPC (1:1) GUVs and LPS-FITC and I follow the FITC fluorescence in membranes. To study and characterize macrodomains in membranes in presence of LPS I grow up GUVs of DMPC pure, DPPC:DOPC (1:1) and DPPC:DOPC:LPS and analize the GP properties of Laurdan.