INTERACTION OF E COLI HEMOLYSIN WITH RED BLOOD CELLS

大肠杆菌溶血素与红细胞的相互作用

• 批准号: 8170981
• 负责人: Laura Bakas
• 金额: $ 3.75万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170981
• 关键词: Animals; Behavior; Cell Shape; Cholesterol; Computer Retrieval of Information on Scientific Projects Database; Cytolysis; Equus caballus; Erythrocytes; Escherichia coli; Funding; Grant; Hemolysin; Institution; Measurement; Microscope; Oryctolagus cuniculus; Proteins; Research; Research Personnel; Resources; Sheep; Source; Testing; Toxin; United States National Institutes of Health; laurdan

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Hemolysin is a toxin of E. Coli that induces lysis of red blood cells from different animal species. Here we test the influence of bilayer composition on protein interaction with red blood cells of sheep, horse and rabbit, and the effect of cholesterol depletion on cells shape and behavior. We analize these parameters by Laurdan General Polarization measurements at the microscope

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 溶血素是大肠杆菌的一种毒素。诱导来自不同动物物种的红细胞溶解的大肠杆菌。在这里，我们测试了双层组成对蛋白质与羊，马和兔的红细胞相互作用的影响，以及胆固醇消耗对细胞形状和行为的影响。我们在显微镜下通过Laurdan广义偏振测量来分析这些参数