INTERACTION OF E COLI HEMOLYSIN WITH RED BLOOD CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 溶血素是一种大肠杆菌毒素，能诱导不同动物物种的红细胞溶解。在这里，我们测试了双层组成对蛋白质与绵羊、马和兔红细胞相互作用的影响，以及胆固醇耗竭对细胞形态和行为的影响。我们通过在显微镜上进行Laurdan一般偏振测量来分析这些参数

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Hemolysin is a toxin of E. Coli that induces lysis of red blood cells from different animal species. Here we test the influence of bilayer composition on protein interaction with red blood cells of sheep, horse and rabbit, and the effect of cholesterol depletion on cells shape and behavior. We analize these parameters by Laurdan General Polarization measurements at the microscope