ANALYSIS OF CELL MIGRATION IN AN AXOLOTL SKIN EXPLANT WOUND MODEL

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们已经开发了一种外植体皮肤模型来研究美西螈皮肤的伤口愈合。使用传统的显微镜，我们已经确定，一个2毫米的伤口repithelializes在不到10小时。我们使用LFD来解决以下问题。 1)使实验伤口床再生的细胞的来源是什么？它们来源于表皮的基底细胞还是基底上细胞？ 2)细胞迁移的速率和方向是什么？这在迁移的表皮中是否有所不同？基质是否影响迁移？

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have developed an explant skin model to study wound healing in axolotl skin. Using conventional microscopy we have established that a 2 mm wound repithelializes in less than 10 hours. We are using the LFD to address the following questions. 1) What is the origin of the cells that repithelialize the experimental wound bed? Are they derived from basal or suprabasal cell in the epidermis? 2) What is the rate and direction of cell migration? Does this vary within the migrating epidermis? Does the substrate affect migration?