STRUCTURE AND DYNAMICS OF 6-THIOGUANINE MODIFIED DNA

这个子项目是众多研究子项目之一

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Toxicity of thiopurines results after being metabolized and incorporated into DNA as 6-thiodeoxyguanosine. The alteration of local DNA structure by thiodeoxyguanosine and/or its metabolite methyl-6-thiodeoxyguanosine alters important DNA/protein interactions including DNA-mismatch repair, topoisomerase II, telomerase, and RNase H. We are using solution NMR spectroscopy to characterize the changes in local DNA structure and dynamics in 6-thioguanine modified oligonucleotides