STRUCTURE AND DYNAMICS OF 6-THIOGUANINE MODIFIED DNA

6-硫鸟嘌呤修饰 DNA 的结构和动力学

• 批准号: 7721640
• 负责人: STEPHEN U DUNHAM
• 金额: $ 0.23万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721640
• 关键词: Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Structure; DNA-Protein Interaction; Funding; Grant; Institution; Mismatch Repair; NMR Spectroscopy; Oligonucleotides; Research; Research Personnel; Resources; Ribonuclease H; Solutions; Source; Structure; Telomerase; Thioguanine; Topoisomerase II; Toxic effect; United States National Institutes of Health; thiopurine

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Toxicity of thiopurines results after being metabolized and incorporated into DNA as 6-thiodeoxyguanosine. The alteration of local DNA structure by thiodeoxyguanosine and/or its metabolite methyl-6-thiodeoxyguanosine alters important DNA/protein interactions including DNA-mismatch repair, topoisomerase II, telomerase, and RNase H. We are using solution NMR spectroscopy to characterize the changes in local DNA structure and dynamics in 6-thioguanine modified oligonucleotides

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 硫嘌呤的毒性在被代谢并作为6-硫代脱氧鸟苷掺入DNA后产生。硫代脱氧鸟苷和/或其代谢物甲基-6-硫代脱氧鸟苷对局部DNA结构的改变改变了重要的DNA/蛋白质相互作用，包括DNA错配修复、拓扑异构酶II、端粒酶和RNA酶H。我们正在使用溶液核磁共振光谱来表征6-硫代鸟嘌呤修饰的寡核苷酸中局部DNA结构和动力学的变化