QUANTIFICATION OF 13C-OPTIMIZED CRYOGENICALLY-COOLED PROBES FOR CHEM RES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 进行测试是为了量化低温冷却探头在化学研究中的效用，特别是在获得直接检测到的碳13数据方面。高度不饱和化合物是威斯康星大学麦迪逊分校化学系各种研究项目的合成目标。这些化合物有时很难用现在复杂化合物的核磁共振分析中常见的反向(质子检测)方法来表征。不依赖于1H-13C J-偶联存在的优秀的完全表征技术，例如不充分，在过去需要13C标记或非常高的浓度。使用低温冷却的探测器获取中等浓度自然丰度化合物的数据的能力，以及13C前置放大器也被冷却以降低其噪声系数的能力，将使用NMRFAM的750 MHz低温探测器进行测试。还将量化探测器对改进1D~(13)C数据获取的效用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Tests are performed to quantify the utility of cryogenically-cooled probes for chemical research, especially with respect to obtaining directly-detected carbon-13 data. Highly unsaturated compounds are synthetic targets for a variety of research projects in the Chemistry Department at the UW-Madison. These compounds are sometimes difficult to characterize using inverse (proton-detected) methods now common with NMR analysis of complex compounds. Excellent techniques for total characterization that do not rely on the presence of 1H-13C J-couplings, such as INADEQUATE, have in the past required either 13C labeling or very high concentrations. The ability to obtain such data for moderately concentrated natural abundance compounds using a cryogenically-cooled probe, with the 13C preamp also cooled to reduce its noise factor, will be tested using NMRFAM's cryoprobe at 750 MHz. The utility of the probe for improving the acquisition of 1D 13C data will also be quantified.