CONCENTRATION STUDIES OF A ROTENONE IN BENZENE-D6

苯 D6 中鱼藤酮的浓度研究

• 批准号: 8168984
• 负责人: CHARLES G FRY
• 金额: --
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168984
• 关键词: Applications Grants; Benzene; Blood capillaries; Carbohydrate Chemistry; Chemistry; Computer Retrieval of Information on Scientific Projects Database; Data; Funding; Future; Grant; Housing; Institution; Pharmacy facility; Research; Research Personnel; Resources; Rotenone; Sampling; Sampling Studies; Screening procedure; Source; United States National Institutes of Health; capillary; combinatorial chemistry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are planning to study samples of varying concentration, ranging from very concentrated (50 mM) to very dilute (0.0032 mM), to determine the benefits of obtaining a cryogenically cooled probe in the NMR facility in the Chemistry department. We have chosen "rotenone in benzene-d6" as our reference compound and will be comparing the results we obtain from ?jaulan' with results acquired on our in house 600 MHz spectrometer. The results will also be compared with data obtained on a "CapNMR" probe (capillary NMR; made by Protasis/MRM) installed on a 500 MHz spectrometer in the Department of Pharmacy, using samples of the same concentration (50 mM and 10 mM), but significantly lower volume (5-10 ?l). The data from these studies will enable us to get a sense of different sensitivities from three types of probes and will be used as preliminary data for future grant applications. The data will also direct particular Chemistry research groups to utilize the spectrometers available at NMRFAM. Our findings will help the Blackwell group, enabling them to obtain NMR data for their combinatorial chemistry project; the Gellman group and their screening compounds in identifying new foldamers; the Kiessling group and their application of NMR studies to carbohydrate chemistry; as well as many others in the Department of Chemistry.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们计划研究不同浓度的样品，从非常浓缩（50 mM）到非常稀释（0.0032 mM），以确定在化学系的NMR设施中获得低温冷却探针的好处。 我们选择“苯-d 6中的鱼藤酮”作为参考化合物，并将比较我们从？jaulan'，其结果在我们的内部600 MHz光谱仪上获得。 还将结果与在药学系安装在500 MHz光谱仪上的“CapNMR”探针（毛细管NMR;由Protasis/MRM制造）上获得的数据进行比较，使用相同浓度（50 mM和10 mM）但体积显著较低（5-10 mM）的样品。l）。 这些研究的数据将使我们能够从三种类型的探针中获得不同的灵敏度，并将用作未来拨款申请的初步数据。 这些数据还将指导特定的化学研究小组利用NMRFAM提供的光谱仪。 我们的研究结果将帮助Blackwell小组，使他们能够获得NMR数据用于他们的组合化学项目; Gellman小组及其筛选化合物以识别新的折叠体; Kiessling小组及其将NMR研究应用于碳水化合物化学;以及化学系的许多其他人。