MECHANISMS OF PROTEIN AND RNA FOLDING AT HIGH RESOLUTION

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this research is to gain insights into the fundamental mechanisms governing protein and RNA folding at high resolution by nuclear magnetic resonance. The protein folding subproject aims at dissecting how chain length affects the specific conformational features of small single domain proteins in solution. The studies will be performed in the absence and presence of cotranslationally active molecular chaperones. Backbone assignments and conformatiion of N-terminal model peptide fragments will be studied by double and triple resonance NMR. The RNA folding subproject addresses fundamental issues such as the presence of kinetic/equilibrium intermediates and base pair formation pathways in small biologically relevant model RNA sequences.