MICRO-CT OF CARDIAC FUNCTION IN THE CD RAT WITH DOBUTAMINE CHALLENGE

多巴酚丁胺挑战 CD 大鼠心脏功能的显微 CT

• 批准号: 7726196
• 负责人: BRAIN BERRIDGE
• 金额: $ 0.65万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7726196
• 关键词: Animals; Biological; Biological Testing; Blood; Calibration; Cardiac; Cardiac Output; Cardiac Volume; Chronology; Clinical; Computer Retrieval of Information on Scientific Projects Database; Data; Dobutamine; EFRAC; End Point; Error Sources; Funding; Generations; Gold; Grant; Image; Institution; Intravenous infusion procedures; Left; Life; Measures; Methods; Myocardium; Noise; Physiology; Protocols documentation; Rattus; Relative (related person); Reporting; Research; Research Design; Research Personnel; Resources; Scanning; Source; Standards of Weights and Measures; Stroke Volume; System; Techniques; Testing; Thick; Time; United States National Institutes of Health; Week; X-Ray Computed Tomography; day

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Study Design -- Animals: approx. 6 week old CD rats (250 gm); n = 6 ---Test article: Dobutamine, 30 ug/kg/min IV infusion Quantitative endpoints- total cardiac volume; left, right, and septal wall thickness; EDV, ESV, ejection fraction, stroke volume, cardiac output, fractional shortening Imaging methods: All imaging will be performed on the first-generation micro-CT system at the Duke CIVM. We will acquire 12 points in the cardiac cycle for 6 animals at two different times separated by at least one week. Each session will include a baseline followed by a challenge with dobutamine. Chronology: 2 animals imaged/day X 3 days ; Animals will be returned to their cages for one week to recover to a baseline physiology and the study will be repeated. Phantom Calibrations: We will validate the CT scanning protocol and analysis using a phantom with objects of accurately known volumes. A (static) phantom will be constructed with CT (Hounsfield) differences comparable to those of the animal (myocardium/blood pool). The volumes of the phantom objects will be know with accuracy of at least 1%. The phantom will be scanned with techniques that produce contrast to noise at least 2X better than that seen in the clinical (live animal) setting. We will digitally alter the image sets to produce images with contrast to noise @ 0.5X, 1.0 X and (original) 2.0 X the CNR. This will allow us to separate any sources of errors from scanning and segmentation from the biological variability we wish to measure. Analysis: Phantom data will analyzed with three independent approaches : --- Autosegmentation using Vitrea cardiac functional analysis --- Autosegmentation using the PSC browser --- Semiautomated segmentation using Image J In all cases, the error will be reported relative to the gold standard (static phantom). At this point we may choose one or both methods to apply to the biological test data.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 研究设计 --动物：大约。6周龄Cd大鼠(250克)；n=6 -测试品：多巴酚丁胺，30ug/kg/min静脉滴注 定量终点-心总容量；左、右和室间隔厚度；EDV、ESV、射血分数、每搏量、心输出量、缩短率 成像方法：所有成像将在Duke CIVM的第一代Micro-CT系统上进行。 我们将在间隔至少一周的两个不同时间为6只动物获得心跳周期中的12分。每次会议将包括一个基线，然后是多巴酚丁胺挑战。 时间顺序：2只动物/天×3天；动物将被送回笼子一周，以恢复到基线生理，该研究将重复进行。 体模校准：我们将使用具有准确已知体积的物体的体模来验证CT扫描协议和分析。将构建一个(静态)体模，其CT(Hounsfield)差异与动物(心肌/血池)的CT差异相当。将以至少1%的精度获知模型对象的体积。体模的扫描技术将产生比临床(活体动物)环境中看到的至少两倍的对比度噪音。我们将对图像集进行数字更改，以产生与噪声对比度为0.5X、1.0X和(原始)2.0X的图像。这将使我们能够将扫描和分割的任何误差来源与我们希望测量的生物可变性分开。 分析：Phantom数据将通过三种独立的方法进行分析： -使用Vitrea心功能分析进行自动分割 -使用PSC浏览器进行自动分段 -使用图像J的半自动分割 在所有情况下，都将相对于黄金标准(静态虚线)报告错误。在这一点上，我们可以选择一种或两种方法来应用于生物测试数据。