PERFUSION MEASUREMENT WITH ULTRASOUND AND MRI OF TUMORS IN MICE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目涉及扫描患有侧腹肿瘤的小鼠，并使用7 T MRI系统和超声系统进行灌注测量。本研究主要旨在了解使用VisualSonics提供的造影剂在超声中进行灌注测量的工作情况，并将这些测量结果与MRI进行比较。该项目将涉及在MRI和超声中使用造影剂。首先使用MRI系统扫描侧腹有肿瘤的动物（N=10），然后将动物移至超声系统。 MRI实验将主要涉及使用T1测量序列，然后使用MR微量注射器在尾静脉中推注Magnevist。将使用3D编码序列（取决于对比度动态）收集肿瘤的动态对比度增强图像。T1测量序列可以是简单的自旋回波序列。或FSE或FLASH型序列。T1测量主要用于对肿瘤中的各种灌注指标进行定量计算。 将使用定制的VisualSonics血流测量程序进行超声灌注测量。微泡将用作造影剂。后分析将包括使用两种成像模式收集的图像的灌注度量的量化。将对灌注指标进行比较。档案系统将需要能够将两种模式的衍生数据存档。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The project involves scanning mice with flank tumors and make perfusion measurements using the 7T MRI system and the ultrasound system. The study is primarily designed to understand the working of the perfusion measurements in ultrasound using the contrast agent provided by VisualSonics and comparing those measurements with MRI. The project will involve the use of contrast agents both in MRI and ultrasound. Animals (N=10) with tumors on the flank will be scanned first using the MRI systems and then the animal will be moved to the Ultrasound system. MRI experiments will primarily involve using a T1 measuring sequence followed by a bolus injection of Magnevist in the tail vein using the MR microinjector. Dynamic contrast enhanced images of the tumor will be collected using a 3D encoding sequence (depending on contrast dynamics). The T1 measuring sequence can be a simple spin-echo sequence. or FSE or a FLASH type sequence. T1 measurements are primarily made to perform quantitative calculations of the various perfusion metrics in the tumors. Perfusion measurements in ultrasound will be made using the custom-built VisualSonics program for blood flow measurements. Microbubbles will be used as the contrast agent. Post-analysis will include the quantification of the perfusing metrics from the images collected using both the imaging modalities. Comparison of the perfusion metrics will be made. Archival system will need to be able to archive derived data from both modalities.