ULTRASTRUCTURAL CHANGES IN DENDRITIC SPINES IN RESPONSE TO CONTROLLED STIMULATIO

树突棘响应受控刺激的超微结构变化

• 批准号: 7722470
• 负责人: Terunaga Nakagawa
• 金额: $ 1.17万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722470
• 关键词: Complex; Computer Retrieval of Information on Scientific Projects Database; Dendritic Spines; Development; Funding; Goals; Grant; Image; Imaging Techniques; Institution; Label; Microscopy; Molecular; Morphology; Preparation; Process; Protocols documentation; Research; Research Personnel; Resolution; Resources; Shapes; Source; Specimen; Structure; Synapses; System; United States National Institutes of Health; Work; electron tomography; method development; reconstruction; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to develop and apply unique specimen preparation protocols and correlated imaging techniques to examine changes in activity-dependent dendritic spine morphology and synaptic ultrastructure.This work will propel development and application of the tetracysteine/biarsenical labeling system for correlated microscopy and of high resolution imaging, shaping development of methods used for processing, refinement and interpretation of information in reconstructions from electron tomography when specifically applied to the complex problem of deducing the molecular organization of synaptic structures.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本项目的目标是开发和应用独特的标本制备方案和相关成像技术，以检查活动依赖性树突棘形态和突触超微结构的变化。这项工作将推动相关显微镜和高分辨率成像的四半胱氨酸/双砷标记系统的开发和应用，形成用于处理的方法的发展，在电子断层扫描的重建中，当专门应用于推导突触结构的分子组织的复杂问题时，对信息的细化和解释。