RETROGRADE SIGNALING IN AXONS AND DENDRITES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 神经元轴突中的逆行信号和树突中的顺行信号在一定程度上是由Pincher介导的大内吞机制引起的。为了描述这一过程，量子点标记的配体或四半胱氨酸标记的重组蛋白将被用来在光学显微镜(荧光)和电子显微镜(TEM)水平上跟踪信号内体的逆行运输。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Retrograde signaling in neuronal axons and anterograde signaling in dendrites ocurrs in part by a Pincher-mediated mechanism of macroendocytosis. To characterize this process, Quantum dot-labeled ligand or tetracysteine-tagged recombinant proteins will be used to follow retrograde transport of signaling endosomes at both the light microscope (fluorescence) and electron microscope (TEM) levels.