RETROGRADE SIGNALING IN AXONS AND DENDRITES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 神经元轴突中的逆行信号和树突中的顺行信号部分通过Pincher介导的巨内吞机制发生。 为了表征该过程，量子点标记的配体或四半胱氨酸标记的重组蛋白将用于在光学显微镜（荧光）和电子显微镜（TEM）水平上追踪信号传导内体的逆行转运。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Retrograde signaling in neuronal axons and anterograde signaling in dendrites ocurrs in part by a Pincher-mediated mechanism of macroendocytosis. To characterize this process, Quantum dot-labeled ligand or tetracysteine-tagged recombinant proteins will be used to follow retrograde transport of signaling endosomes at both the light microscope (fluorescence) and electron microscope (TEM) levels.