CORE--CELL ANALYSIS FACILITY

核心——细胞分析设施

• 批准号: 7417852
• 负责人: JENNIFER D. BLACK
• 金额: $ 15.18万
• 依托单位: ROSWELL PARK CANCER INSTITUTE CORP
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7417852
• 关键词: Animals; Apoptosis; Applications Grants; Arts; Autopsy; Autoradiography; Cancer Center Support Grant; Cells; Complement; Confocal Microscopy; Contract Services; Cryoultramicrotomy; Custom; DAPI; Data; Detection; Development; Doctor of Philosophy; Electron Microscope; Electron Microscopy; Equipment; Experimental Designs; Fee-for-Service Plans; Frozen Sections; Funding; Goals; Histology; Immunofluorescence Microscopy; Immunohistochemistry; In Situ; In Situ Hybridization; Individual; Instruction; Investments; Knockout Mice; Laboratories; Light; Methodology; Microscope; Microscopy; Nature; Negative Staining; Nuclear; Organelles; Paraffin; Paraffin Embedding; Peer Review; Phenotype; Platinum; Preparation; Primary Cell Cultures; Process; Proliferating; Publications; Recruitment Activity; Research; Research Personnel; Research Project Grants; Resolution; Resources; Scanning; Services; Shadowing (Histology); Source; Staining method; Stains; Strategic Planning; Students; System; Techniques; Tissues; Toxicology; Training; Transgenic Organisms; base; cost; immunocytochemistry; instrumentation; interest; light microscopy; member; method development; monolayer; programs; tissue processing; transmission process

The Cell Analysis Resource provides morphological services at the light and electron microscope (EM) levels of resolution and assists with experimental design and data interpretation. The Resource is supervised by Jennifer Black, PhD, and staffed by three technicians. Capabilities include transmission and scanning EM, confocal microscopy, frozen sectioning and cryoultramicrotomy, paraffin embedding, sectioning and staining, as well as specialized techniques such as pre- and post-embedding immunocytochemistry, immunohistochemistry on paraffin sections, immunofluorescence microscopy, scanning immuno-EM, quantitative EM autoradiography, and in situ hybridization. The facility is equipped with the instrumentation required to perform these functions in an efficient and cost-effective manner. Major equipment recently purchased includes a Leica TCS SP2 Spectral Confocal Microscope. A key value-added component is custom methods development in a variety of highly specialized, labor-intensive morphological techniques and approaches, services not readily obtained from outside sources. Demand for the services of the Resource is high and increasing. Instruction in morphological techniques and use of appropriate instrumentation is provided. During a recent 12-month period, the Resource contributed to the research of 35 senior investigators from all 5 CCSG Programs. 98% of overall Resource utilization is by Program members with peer-reviewed funding. Studies performed during the project period (1999-2002) supported 65 research grants, and provided data included in 84 publications, approximately 40% of which were co-authored by more than one Program member. The Resource contributed to 19 new/renewal grant applications and 18 pending applications. A chargeback system is used for routine services, and fees for long-term specialized services are arranged on an individual basis. The Strategic Plan emphasizes continuing to provide state-of-the-art morphological information at both the light and EM levels of resolution at a reasonable cost, and to support RPCI's new recruits whose research will require and benefit from morphological correlation.

细胞分析资源提供光学和电子显微镜的形态学服务 (EM)分辨率水平，并协助实验设计和数据解释。该资源由Jennifer Black博士监督，并配备了三名技术人员。能力包括透射和扫描EM、共聚焦显微镜、冷冻切片和冷冻切片、石蜡包埋、切片和染色，以及包埋前和包埋后免疫细胞化学、石蜡切片免疫组织化学、免疫荧光显微镜、扫描免疫EM、定量EM放射自显影和原位杂交等专业技术。该设施配备了以高效和具有成本效益的方式履行这些职能所需的仪器。最近购买的主要设备包括Leica TCS SP2光谱共聚焦显微镜。一个关键的增值 这一组成部分是在各种高度专业化的、劳动密集型的形态学技术和方法方面的定制方法开发，这些服务不容易从外部来源获得。对资源服务的需求很高，而且还在增加。提供了形态学技术和使用适当仪器的指导。在最近的12个月期间，该资源为来自所有5个CCSG计划的35名高级研究人员的研究做出了贡献。98%的整体资源利用是由计划成员通过同行评审的资金。在项目期间（1999-2002年）进行的研究支持了65项研究赠款，并提供了84篇出版物中包含的数据，其中约40%是由一个以上的计划成员共同撰写的。该资源为19项新的/延长的赠款申请和18项 待处理的申请。常规服务采用收费制度，长期专业服务的费用按个人情况安排。该战略计划强调继续以合理的成本提供最先进的形态学信息，包括光和EM分辨率，并支持RPCI的新成员，他们的研究将需要并受益于形态学相关性。