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Lymphocyte Culture Core

淋巴细胞培养核心

基本信息

批准号：
7726752
负责人：
William M Sutherland
金额：
$ 3.86万
依托单位：
UNIVERSITY OF VIRGINIA
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7726752
关键词：
Affinity Affinity Chromatography Animal Experimentation Animals Antibodies Antigens Ascites Attention Avidity Basic Science Biological Assay Bioreactors Cancer Center Cell Line Cell fusion Chromatography Cloning Collection Cryopreservation Cryopreserved Cell Cultured Cells Enzyme-Linked Immunosorbent Assay Equipment and supply inventories Facility Construction Funding Category Foundations Freezing G-substrate Hybridomas Immunization In Vitro Individual Injection of therapeutic agent Intellectual Property Laboratories Legal patent Licensing Lymphocyte Lymphocyte Function Monoclonal Antibodies Multiple Myeloma Numbers Production Protocols documentation Reagent Recombinant Proteins Recovery Research Personnel Research Support Schedule Screening procedure Serum Services Shared Resource Cancer Center Shipping Ships System Technology Titrations Universities Virginia base cryogenics hybridoma production medical schools member monoclonal antibody production polyclonal antibody programs repository

项目摘要

The Lymphocyte Culture Core (LCC), a hybridoma facility, is a research support facility of the University of Virginia School of Medicine and the Cancer Center. The primary function of the LCC is to make available to researchers the most current technology and expertise for the construction and selection of lymphocytemyeloma hybridomas for the production of monoclonal antibodies. The LCC also provides expertise in the use of these reagents in basic research programs. Services are customized to the specific requirements of individual investigators to optimize the recovery of appropriate antigen specific monoclonal antibodies. Initial discussions are held with the investigator and his staff in order to understand the experimental system employed in the laboratory and to determine the specific applications for which monoclonal antibodies are required. The staff of the LCC then plans and executes all aspects of hybridoma construction and selection and monoclonal antibody production. Specifically, the LCC provides approved animal experimentation protocols for all aspects of monoclonal antibody production, including: immunizations, sera collection, intrasplenic injection and ascites production. Center staff immunize animals, collect sera, perform all cell culture aspects of hybridoma construction and selection, and develop ELISA assay strategies appropriate for sera titration and for screening large numbers of hybridoma culture supernatants. Once an appropriate assay system has been developed and used to demonstrate the successful immunization of the necessary animals, fusions are scheduled on a first come first served basis. The center conducts all aspects of cell fusion, assaying by ELISA for specific antibody positive cultures, culturing, cloning, freezing, and recovery of specific antibody producing clones. Investigators receive, upon request, hybridoma culture supernatants and individual hybridoma clones. The center also provides cryopreservation, storage and recovery of cryopreserved cells. Adjunct services include monoclonal antibody isotyping and sub-isotyping, bulk monoclonal antibody production in vitro using disposable bioreactors, ascites production, monoclonal and polyclonal antibody purification by affinity chromatography on recombinant Protein G columns, and antibody inventory, storage and shipping. The LCC also serves as a repository, production facility and distribution point for intellectual property (hybridomas and monoclonal antibodies) licensed by the University of Virginia Patent Foundation.

淋巴细胞培养核心 (LCC) 是一个杂交瘤设施，是马里兰大学的一个研究支持设施弗吉尼亚医学院和癌症中心。 LCC 的主要职能是为研究人员利用最新的技术和专业知识来构建和选择淋巴细胞骨髓瘤用于生产单克隆抗体的杂交瘤。 LCC 还提供以下方面的专业知识：这些试剂在基础研究项目中的使用。服务是根据特定要求定制的个别研究人员优化适当抗原特异性单克隆抗体的回收率。最初的与研究者及其工作人员进行讨论，以了解实验系统在实验室中使用并确定单克隆抗体的具体应用必需的。然后，LCC 的工作人员计划并执行杂交瘤构建和选择的各个方面和单克隆抗体的生产。具体来说，LCC 为单克隆抗体的各个方面提供经批准的动物实验方案抗体生产，包括：免疫、血清采集、脾内注射和腹水生产。中心工作人员对动物进行免疫、收集血清、执行杂交瘤构建的所有细胞培养方面以及选择，并开发适合血清滴定和筛选大量的 ELISA 测定策略杂交瘤培养上清液。一旦开发出适当的测定系统并用于证明必要的动物已成功免疫，融合按先到先得的顺序安排先服务的基础上。中心进行细胞融合的各个环节，通过ELISA检测特异性抗体阳性培养、培养、克隆、冷冻和回收产生特异性抗体的克隆。研究人员根据要求收到杂交瘤培养物上清液和单个杂交瘤克隆。这中心还提供冷冻细胞的冷冻保存、储存和回收。辅助服务包括单克隆抗体同种分型和亚同种分型，使用体外批量单克隆抗体生产一次性生物反应器、腹水产生、单克隆和多克隆抗体亲和纯化重组蛋白 G 柱色谱，以及抗体库存、储存和运输。这 LCC 还充当知识产权的存储库、生产设施和分发点（杂交瘤和单克隆抗体）由弗吉尼亚大学专利基金会许可。