Lymphocyte Culture Core

淋巴细胞培养核心(LCC)是一种杂交瘤设施，是加州大学的研究支持设施。 弗吉尼亚医学院和癌症中心。LCC的主要功能是使 研究人员构建和选择淋巴细胞性骨髓瘤的最新技术和专业知识 用于生产单抗的杂交瘤。LCC还提供以下方面的专业知识 这些试剂在基础研究项目中的使用。服务是根据以下特定要求定制的 个体研究人员优化回收合适的抗原特异性单抗。首字母 为了了解实验系统，与调查员和他的工作人员进行了讨论。 在实验室中使用，并确定单抗的具体应用 必填项。然后，LCC的工作人员计划并执行杂交瘤构建和选择的所有方面 和单抗的产生。 具体地说，LCC为克隆的所有方面提供了经批准的动物实验方案 抗体产生，包括：免疫、血清采集、脾内注射和腹水产生。 中心工作人员为动物接种疫苗，收集血清，进行杂交瘤构建的所有细胞培养方面的工作，以及 选择和开发适用于血清滴定和大量筛查的酶联免疫吸附试验方法 杂交瘤培养上清液。一旦开发了适当的检测系统并用于 展示成功免疫的必要动物，融合计划先到先得 先到先得。该中心进行细胞融合的所有方面，通过酶联免疫吸附试验检测特定的抗体 阳性培养、培养、克隆、冷冻和回收产生特异性抗体的克隆。 调查人员应要求收到杂交瘤培养上清和单个杂交瘤克隆。这个 该中心还提供冷冻保存、储存和恢复冷冻保存的细胞。附属服务 包括单抗等位分型和亚等位分型、体外生产大量单抗 一次性生物反应器、腹水生产、单抗和多克隆抗体亲和纯化 重组蛋白G柱上的层析以及抗体的库存、储存和运输。这个 LCC也是知识产权的储存库、生产设施和分发点 (杂交瘤和单抗)获得弗吉尼亚大学专利基金会的许可。

The Lymphocyte Culture Core (LCC), a hybridoma facility, is a research support facility of the University of Virginia School of Medicine and the Cancer Center. The primary function of the LCC is to make available to researchers the most current technology and expertise for the construction and selection of lymphocytemyeloma hybridomas for the production of monoclonal antibodies. The LCC also provides expertise in the use of these reagents in basic research programs. Services are customized to the specific requirements of individual investigators to optimize the recovery of appropriate antigen specific monoclonal antibodies. Initial discussions are held with the investigator and his staff in order to understand the experimental system employed in the laboratory and to determine the specific applications for which monoclonal antibodies are required. The staff of the LCC then plans and executes all aspects of hybridoma construction and selection and monoclonal antibody production. Specifically, the LCC provides approved animal experimentation protocols for all aspects of monoclonal antibody production, including: immunizations, sera collection, intrasplenic injection and ascites production. Center staff immunize animals, collect sera, perform all cell culture aspects of hybridoma construction and selection, and develop ELISA assay strategies appropriate for sera titration and for screening large numbers of hybridoma culture supernatants. Once an appropriate assay system has been developed and used to demonstrate the successful immunization of the necessary animals, fusions are scheduled on a first come first served basis. The center conducts all aspects of cell fusion, assaying by ELISA for specific antibody positive cultures, culturing, cloning, freezing, and recovery of specific antibody producing clones. Investigators receive, upon request, hybridoma culture supernatants and individual hybridoma clones. The center also provides cryopreservation, storage and recovery of cryopreserved cells. Adjunct services include monoclonal antibody isotyping and sub-isotyping, bulk monoclonal antibody production in vitro using disposable bioreactors, ascites production, monoclonal and polyclonal antibody purification by affinity chromatography on recombinant Protein G columns, and antibody inventory, storage and shipping. The LCC also serves as a repository, production facility and distribution point for intellectual property (hybridomas and monoclonal antibodies) licensed by the University of Virginia Patent Foundation.