Gene Targeting & Transgenic Facility

基因打靶

• 批准号: 7726757
• 负责人: WENHAO XU
• 金额: $ 1.58万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7726757
• 关键词: Animal Model; Animals; Complex; Derivation procedure; ES Cell Line; Embryo; Engineering; Ensure; Event; Facility Designs; Gene Targeting; Genes; Human; Image; Institution; Knock-out; Malignant Neoplasms; Mission; Mus; Mutant Strains Mice; Oncogenic; Positioning Attribute; Process; Production; Reproduction; Research Personnel; Resources; Services; Targeted Research; Technology; Testing; Transgenic Mice; Transgenic Organisms; Tumor Suppressor Genes; Universities; Virginia; anticancer research; design; embryonic stem cell; human cancer mouse model; model development

netically engineered animal models for biomedical studies of human cancer for researchers at UVa and their collaborators in other academic institutions. The GTTF's mission is to support transgenic and gene targeting research endeavors, to ensure the most advanced technologies are available and to serve as a resource for these technologies. GTTF provides the following services: (1) transgenic mouse production; (2) chimeric mouse production; (3) gene targeting in embryonic stem cells; (4) transgenic embryo cryopreseration. These services are designed to expedite the process of animal model development and conservation, and maximize the use of resources. As a result of the facility's reorganizing efforts, the new director took over the position August 1, 2005. In order to expand the facility's service capabilities, the new director is in the process of establishing the following new services: (1) mouse ES cell line derivation; (2) mutant mouse assisted reproduction. The transgenic and gene targeting technology provides a powerful approach for cancer research. The use of transgenic and knockout mouse models of human cancer has proved invaluable for elucidating the functions of oncogenes and tumor suppressor genes, testing targeted therapies and imaging agents, and investigating complex oncogenic events in the whole animal.

为UVA和UVA的研究人员提供用于人类癌症生物医学研究的新工程动物模型 他们在其他学术机构的合作者。 GTTF的使命是支持转基因和基因靶向研究努力，以确保最大 先进的技术是可用的，并且可以作为这些技术的资源。 GTTF提供以下服务：(1)转基因小鼠生产；(2)嵌合体小鼠生产； (3)胚胎干细胞的基因打靶；(4)转基因胚胎的冷冻保存。这些服务是 旨在加快动物模型的开发和保护进程，并最大限度地利用 资源。由于工厂的重组工作，新主任于8月1日上任， 2005年。为了扩大设施的服务能力，新主任正在建立 提供以下新服务：(1)小鼠ES细胞系的建立；(2)突变小鼠辅助生殖。 转基因和基因打靶技术为癌症研究提供了强有力的手段。这个 事实证明，使用人类癌症的转基因和基因敲除小鼠模型对于阐明 癌基因和肿瘤抑制基因的功能，测试靶向治疗和显像剂，以及 在整个动物中研究复杂的致癌事件。