Gene Targeting & Transgenic Facility

基因打靶

• 批准号: 8104155
• 负责人: WENHAO XU
• 金额: $ 3.09万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8104155
• 关键词: Animal Model; Animals; Complex; Derivation procedure; ES Cell Line; Embryo; Engineering; Ensure; Event; Facility Designs; Gene Targeting; Human; Image; Institution; Knockout Mice; Malignant Neoplasms; Mission; Mus; Mutant Strains Mice; Oncogenic; Positioning Attribute; Process; Production; Research Personnel; Resources; Services; Suppressor Genes; Targeted Research; Technology; Testing; Transgenic Mice; Transgenic Organisms; Tumor Suppressor Genes; Universities; Virginia; animal model development; anticancer research; assisted reproduction; design; embryonic stem cell; mouse model

netically engineered animal models for biomedical studies of human cancer for researchers at UVa and their collaborators in other academic institutions. The GTTF's mission is to support transgenic and gene targeting research endeavors, to ensure the most advanced technologies are available and to serve as a resource for these technologies. GTTF provides the following services: (1) transgenic mouse production; (2) chimeric mouse production; (3) gene targeting in embryonic stem cells; (4) transgenic embryo cryopreseration. These services are designed to expedite the process of animal model development and conservation, and maximize the use of resources. As a result of the facility's reorganizing efforts, the new director took over the position August 1, 2005. In order to expand the facility's service capabilities, the new director is in the process of establishing the following new services: (1) mouse ES cell line derivation; (2) mutant mouse assisted reproduction. The transgenic and gene targeting technology provides a powerful approach for cancer research. The use of transgenic and knockout mouse models of human cancer has proved invaluable for elucidating the functions of oncogenes and tumor suppressor genes, testing targeted therapies and imaging agents, and investigating complex oncogenic events in the whole animal.

为弗吉尼亚大学的研究人员设计了用于人类癌症生物医学研究的基因工程动物模型， 他们在其他学术机构的合作者。 GTTF的使命是支持转基因和基因靶向研究工作，以确保 先进的技术是可用的，并作为这些技术的资源。 GTTF提供以下服务：（1）转基因小鼠生产;（2）嵌合小鼠生产; (3)胚胎干细胞基因打靶技术;（4）转基因胚胎冷冻保存技术。这些服务 旨在加快动物模型开发和保护的过程，并最大限度地利用 资源由于该设施的重组努力，新主任接管了8月1日的立场， 2005.为了扩大该设施的服务能力，新主任正在建立 以下新服务：（1）小鼠ES细胞系衍生;（2）突变小鼠辅助生殖。 转基因和基因打靶技术为肿瘤研究提供了一条强有力的途径。的 使用人类癌症的转基因和基因敲除小鼠模型已被证明对于阐明 癌基因和肿瘤抑制基因的功能，测试靶向治疗和显像剂，以及 研究整个动物的复杂致癌事件。