ANALYTICAL IMAGING FACILITY SHARED RESOURCE

分析成像设施（AIF）是一个全面的显微镜共享资源，使常规 和复杂的成像技术提供给整个AECC社区。其中包括宏观成像 使用体视镜，经典显微镜，包括明场、暗场、相衬、Nomarski微分 干涉对比度和落射荧光，通过活细胞成像和细胞操作与光， 标准透射和扫描电子显微镜，高达大分子和整体的cryoEM 安装细胞。大范围的放大率允许研究动物模型。荧光标记细胞 报告基因可以在整个动物或整个器官水平下成像到单个细胞或细胞 隔间该设施为电子显微镜提供定制的全方位样品制备服务， 通过化学固定、树脂包埋和切片、负染、免疫金标记， 临界点干燥和金属遮蔽。此外，该设施还提供全方位的低温 电子显微镜技术，包括通过插入在毫秒范围内快速冷冻，金属镜 或高压冷冻。随后冷冻的样品可以被冷冻替换并在低温下包埋。 温度，深蚀刻和旋转阴影，冷冻断裂或冷冻切片。 该设施传统上强调教导新用户，在个人的基础上，成像 他们需要成功执行实验的技术。工作人员协助用户进行实验 设计、数据收集、定量分析和演示。新手用户在特定的培训 成像技术适合于解决特定的研究目标。有经验的用户可以 利用现有的大量光学器件和附件，定制任何成像站 AIF为基因组成像和组织病理学的显微镜要求提供支持 共享资源。AIF的一个重要资源是新成立的创新实验室， 学院的生物光子学中心，专注于新技术的发展， 随后通过这一共享资源提供给AECC调查人员。自上次CCSG以来 审查了这一共享资源的重要新增设备和服务， 大大增强了AECC的成像能力。

The Analytical Imaging Facility (AIF) is a comprehensive microscopy shared resource that makes routine and complex imaging technologies available to the entire AECC community. These include macro-imaging with a stereoscope, classical microscopy including brightfield, darkfield, phase contrast, Nomarski differential interference contrast and epifluorescence, through live cell imaging and cell manipulation with light, through standard transmission and scanning electron microscopy, up to cryoEM of macromolecules and whole mount cells. The wide range of magnifications allows studying animal models. Cells labeled with fluorescent reporter genes may be imaged at the whole animal or whole organ level down to single cells or cell compartments. The facility offers customized full service sample preparation for electron microscopy ranging from chemical fixation, embedding in resin and ultrathin sectioning, negative staining, immunogold labeling, critical point drying and metal shadowing. In addition, the facility offers a full range of low temperature techniques for electron microscopy including quick freezing in the millisecond range by plunge, metal mirror or high pressure freezing. Subsequently frozen samples can be freeze-substituted and embedded at low temperatures, deep etched and rotary shadowed, freeze fractured or ultrathin cryosectioned. The facility has traditionally emphasized teaching new users, on an individual basis, the imaging techniques they require for successful execution of their experiments. Staff assists users in experimental design, data collection, quantitative analysis and presentation. Novice users are trained in the specific imaging technology appropriate to address the specific research objective. Experienced users may customize any imaging station, utilizing the large inventory of optics and accessories that are available The AIF provides support for the microscopy requirements of the Genome Imaging and Histopathology Shared Resources. An important resource for the AIF is the newly established Innovation Laboratory within the College's Biophotonics Center that is focused on the development of new technologies which are subsequently made available to AECC investigators through this shared resource. Since the last CCSG review important new additions of equipment and services have been made to this shared resource, which have substantially enhanced the imaging capabilities at AECC.