Gene Expression

基因表达

• 批准号: 7826835
• 负责人: LESLEYANN HAWTHORN
• 金额: $ 19.22万
• 依托单位: ROSWELL PARK CANCER INSTITUTE CORP
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7826835
• 关键词: Algorithms; Alleles; Alternative Splicing; Arts; Award; Bioinformatics; Biometry; Books; Budgets; Buffaloes; Cancer Center Support Grant; Categories; Cells; Chromosomal Loss; Chromosome abnormality; Chromosomes; Collaborations; Communities; Complex; Computer software; Consultations; CpG Islands; Custom; DNA; DNA Sequence; DNA-Protein Interaction; Data; Data Analyses; Data Set; Detection; Development; Disease; Doctor of Philosophy; Electrophoresis; Emerging Technologies; Ensure; Event; Exons; Funding; Gene Expression; Gene Expression Microarray Analysis; Gene Expression Profiling; Generations; Genes; Genetic; Genetic Polymorphism; Genome; Genomics; Genotype; Glass; Gold; Grant; Hawthorn plant; Head; Human; Individual; Investigation; Karyotype determination procedure; Label; Loss of Heterozygosity; Maintenance; Maps; Methylation; Microarray Analysis; Mutation; Mutation Analysis; Nucleotides; Oligonucleotides; Organism; Overlapping Genes; Peer Review; Pharmaceutical Preparations; Polymorphism Analysis; Population; Positioning Attribute; Preparation; Procedures; Process; Publications; Quality Control; RNA; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Rewards; Role; Roswell Park Cancer Institute; Running; Sampling; Scanning; Scheme; Sequence Analysis; Services; Site; Solutions; Specificity; Spectrophotometry; Staining method; Stains; Surface; System; Technology; Testing; Time; Tissues; Training; Training Support; Transcript; Translating; Universities; Update; Validation; Variant; Virus; Work; base; chromatin immunoprecipitation; cost; density; design; gel electrophoresis; genetic association; genetic linkage analysis; genome wide association study; genome-wide; innovation; instrument; member; mitochondrial genome; new technology; novel; programs; promoter; research study; response; success; tool; validation studies

The Gene Expression Resource (GER) was established with the successful recruitment of Lesleyann Hawthorn, PhD in 2001. The Resource has flourished since that time with a broad base of satisfied users whose continued patronage is derived from a unique approach to microarray experimentation. Its success is largely due to a commitment to provide researchers with high-quality microarray data with the added advantage of data analysis so that the results provided to the individual researchers are in a usable format, unlike most array-based facilities. Dr Hawthorn and the GER staff keep abreast of emerging technologies to provide users with a broad range of expertise and options. This success is substantiated by a large user base, publications and awarded grants. The Resource is functionally divided into Gene Expression, Genotyping, and Copy Number Analysis at the genomics level. At the level of individual chromosomal regions or specific genes, it offers mutation/polymorphism analysis, SNP validation and discovery, expression level quantification and analysis of methylation status. Most importantly, the Resource offers data analysis for all the platforms that it supports and provides expertise, time and funding for the development of novel technologies. For example, Exon array analysis has been developed which allows simultaneous detection of gene expression and alternative splicing events. Furthermore, the Resource is now able to run Whole-Genome Tiling arrays, which permit the highest-resolution Chip-on-Chip analysis as well as the detection of novel transcripts in genomic DNA. During the last few years, the Resource has been working on innovative approaches to the analysis of genomics data. One of these centers on the high-density SNP arrays that offer the highest density genotyping, as well as simultaneous CGH analysis with accompanying LOH analysis. Gene Expression has been using these arrays to look for gains and losses of chromosomal regions (CGH). The ability of the SNP Mapping arrays to detect standard cytogenetic abnormalities represents a major advancement over conventional karyotyping. The GER has collaborated with Dr Cowell's group (GN) to develop statistical analysis tools that permit the overlay of gene expression data with aCGH data, thus enhancing the power of both these technologies. Members of the six CCSG programs utilized this Resource over the last project period. The Resource was instrumental in enhanced peer-reviewed funding, publications and recruitment efforts. It is anticipated that with the new recruitment into the CSBT Program (Dr Gudkov), MTET Program (Dr Adjei), and Til (Dr Lee) the utilization of the Resource will expand. The Resource is used by all six Programs and 98% of users are CCSG members. $80,355 in CCSG support is requested, representing 11% of the total operating budget.

基因表达资源库（Gene Expression Resource， GER）的建立是由于成功募集了