Resonance Aided Insertion Lancet for serial blood sampling in age-related studies

共振辅助插入柳叶刀用于年龄相关研究中的连续血液采样

• 批准号: 7999435
• 负责人: Ryan S Clement
• 金额: $ 15.67万
• 依托单位: ACTUATED MEDICAL, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2012-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7999435
• 关键词: Age; Aging; Aging-Related Process; Alzheimer' s Disease; Animal Testing; Animals; Antibodies; Area; Automobile Driving; Beginning of Life; Behavior; Behavioral; Biochemical; Biological; Biological Assay; Biological Markers; Blood Chemical Analysis; Blood specimen; Cancer Immunology Science; Cells; Corticosterone; Cross-Sectional Studies; Development; Devices; Disease; Ensure; Exhibits; Frequencies; Generations; Goals; Hand; Heating; Histology; Hormones; In Situ; Individual; Kinetics; Lead; Link; Longevity; Longitudinal Studies; Manuals; Medical; Medical Device; Metabolism; Needles; Neurobiology; Pain; Parkinson Disease; Performance; Phase; Physiological; Pilot Projects; Play; Procedures; Production; Proteins; Psyche structure; Puncture procedure; Rattus; Research; Research Design; Research Personnel; Resort; Risk Management; Rodent; Rodent Model; Role; Safety; Sampling; Sampling Studies; Small Business Innovation Research Grant; Solutions; Stress; Tail; Techniques; Technology; Temperature; Testing; Tissues; Tube; United States National Institutes of Health; Veins; Venipunctures; Work; age difference; age related; design; experience; improved; innovation; insight; nutrition; operation; programs; prototype; public health relevance; research and development; tool; vibration

DESCRIPTION (provided by applicant): Rodent studies have provided significant insights into the general aging process and age-related topics such as nutrition, behavior, immunology, cancer, and neurobiology. In such studies, concentrations of hormones, proteins, antibodies, and other metabolites and their associated kinetics are popular biomarkers because they vary with age and disease state. Typically biochemical assays are performed on blood samples collected following the temporary puncture of a large target vein. Standard blood sampling procedures can increase stress hormone release which may confound blood chemistry analysis, as well as cause pain and discomfort. This hormone release is especially problematic when serial samples are to be obtained over the lifespan. Minimally- perturbing blood sampling techniques are needed that enable studies of hormone fluctuations over the lifespan of individual animals. In this NIH SBIR program, Piezo Resonance Innovations Inc. (PRII) will demonstrate the feasibility of the Resonance Aided Insertion Lancet, an active device specifically designed to reduce the insertion forces required to perform tail venipuncture. In a pilot study conducted by PRII, a prototype was used to obtain blood samples from the tail vein of rats. The study suggested that blood samples were obtained with less stress according to behavioral indicators and corticosterone levels, compared to that of conventional manual lancet insertion. The goals of the Phase I proposal is to determine the parameters for the optimal prototype design, and conduct a more comprehensive set of bench and animal tests to ensure safety and efficacy. The proposed work will lead to the development of a new tool that minimizes animal stress during serial blood samples reducing the number of animals needed per investigational study - animal can survive entire study. This results in fewer animals needed per study, and improved humane treatment of the animals. PUBLIC HEALTH RELEVANCE: Understanding the physiological and biological mechanisms of aging and the role hormones play in healthy and abnormal aging continues to be the focus of much research. Studies of hormone levels in rodent models have been critical to this effort, but the effect of the stress and pain associated with traditional blood sampling techniques is problematic. New minimally-perturbing blood sampling strategies, such as the device to be developed here, are highly desired to enable serial blood sampling over the lifespan to better explore links between changing hormone levels and the aging process.

描述（由申请人提供）：啮齿动物研究为一般衰老过程和与年龄相关的主题提供了重要的见解，如营养，行为，免疫学，癌症和神经生物学。在这些研究中，激素、蛋白质、抗体和其他代谢物的浓度及其相关动力学是流行的生物标志物，因为它们随年龄和疾病状态而变化。通常，对在大的目标静脉的临时穿刺之后收集的血液样品进行生化测定。标准的血液采样程序会增加应激激素的释放，这可能会混淆血液化学分析，并导致疼痛和不适。当要在整个生命周期内获得系列样品时，这种激素释放尤其成问题。需要最小干扰的血液取样技术，以使研究个体动物寿命期间的激素波动成为可能。在这个NIH SBIR计划中，Piezo Resonance Innovations Inc.（PRII）将证明共振辅助穿刺针的可行性，这是一种专门设计用于减少进行尾部静脉穿刺所需插入力的有源器械。在PRII进行的一项试点研究中，使用原型从大鼠尾静脉采集血样。该研究表明，与传统的手动刺血针插入相比，根据行为指标和皮质酮水平，血液样本的压力较小。第一阶段提案的目标是确定最佳原型设计的参数，并进行更全面的实验室和动物试验，以确保安全性和有效性。拟议的工作将导致开发一种新的工具，最大限度地减少动物在连续血液样本的压力，减少每项研究所需的动物数量-动物可以在整个研究中存活。这导致每次研究所需的动物更少，并改善了动物的人道待遇。 公共卫生相关性：了解衰老的生理和生物学机制以及激素在健康和异常衰老中的作用仍然是许多研究的焦点。啮齿动物模型中激素水平的研究对这一努力至关重要，但与传统血液采样技术相关的压力和疼痛的影响是有问题的。新的最小干扰血液采样策略，如这里要开发的设备，是非常需要的，以使连续血液采样在整个生命周期，以更好地探索变化的激素水平和衰老过程之间的联系。

项目成果

Effects of Axial Vibration on Needle Insertion into the Tail Veins of Rats and Subsequent Serial Blood Corticosterone Levels.

轴向振动对大鼠尾静脉针插入及随后连续血皮质酮水平的影响。

• 发表时间: 2016
• 期刊: Journal of the American Association for Laboratory Animal Science : JAALAS
• 作者: Clement,RyanS;Unger,EricaL;Ocón-Grove,OlgaM;Cronin,ThomasL;Mulvihill,MaureenL
• 通讯作者: Mulvihill,MaureenL