Imaging Core

细胞黏附和迁移机制已被认为是调节命运的关键因素 和最多输血血细胞的功能。因此，细胞黏附特性的直接可视化和 影响这些性质的精细结构特征是我们理解输血方式的核心 细胞参与炎症、止血、血栓形成和免疫。成像核心已经被 根据PPG调查人员的明确需求制定的，旨在利用相当大的 联席董事在设计和提供专业设备方面的建议和经验 对细胞黏附分子在输血生物学中的作用进行尖端成像研究。 成像核心的具体目标如下： 1.为活体显微镜的实施和分析提供指导、设备和协助 小鼠组织中黏附和迁移的研究。目前可用的型号包括：Cremaster 肌肉；耳朵皮肤；骨髓；肝脏；窝窝和腹股沟淋巴结；膀胱；膝关节；和小 肠，包括肠系膜、Peyer‘s补片和固有层。 2.为扫描和透射式电子显微镜提供指导、设备和协助 研究，包括专门的样品制备、免疫金标记、高分辨率成像和 数据解释。 3.提供以多光子显微镜为基础的内部和外部记录的途径和专业知识 血管外血细胞黏附、迁移和细胞-细胞相互作用多达六个维度(即 空间、时间、颜色、荧光强度)。 4.为体内血细胞归巢的规划、实施和分析提供指导和帮助 使用已定义的炎症模型。 成像核心将提供的服务将允许参与的调查人员探索如何定义 黏附途径影响血细胞与环境的相互作用，其分辨率从单一的 分子传给完好的动物。

Cellular adhesion and migration mechanisms have been recognized as crucial elements regulating the fate and function of most transfused blood cells. As such, the direct visualization of cell adhesion properties and the fine structural feature that influence these properties is central to our understanding of how transfused cells participate in inflammation, hemostasis, thrombosis and immunity. The Imaging Core has been formulated based on a stated need of PPG investigators and is designed to draw on the considerable experience of its co-directors to provide advice, expertise and specialized equipment in the design and execution of cutting-edge imaging studies on the role of cell adhesion molecules in transfusion biology. The Specific Aims of the Imaging Core are as follows: 1. To provide instruction, equipment and assistance in the execution and analysis of intravital microscopybased adhesion and migration studies in murine tissues. Currently available models include: cremaster muscle; ear skin; bone marrow; liver; popliteal and inguinal lymph node; bladder; knee joint; and small intestine, including mesentery, Peyer's patches and lamina propria. 2. To provide instruction, equipment and assistance for scanning and transmission electron microscopy studies, including specialized sample preparation, immunogold labeling, high resolution imaging, and data interpretation. 3. To provide access to and expertise in multi-photon microscopy-based recording of intra- and extravascular blood cell adhesion, migration and cell-cell interactions in as many as six dimensions (i.e. space, time, color, fluorescence intensity). 4. To provide instruction and assistance in the planning, execution and analysis of blood cell homing in vivo employing defined models of inflammation. Services to be provided by the Imaging Core will allow participating investigators to explore how defined adhesion pathways influence blood cells interactions with their environment at a resolution ranging from single molecules to intact animals.