Sympathochromaffin cell culture

交感嗜铬细胞培养

• 批准号: 7844961
• 负责人: Vivian Y. H Hook
• 金额: $ 24.11万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7844961
• 关键词: Adrenal Glands; Adrenal Medulla; Blood Pressure; Cattle; Cell Culture System; Cell Culture Techniques; Cell Line; Cells; Data; Exocytosis; Forskolin; Genes; Goals; Human; Hypertension; Molecular Genetics; Mus; Neuroeffector Junction; Neuroendocrine Cell; Nicotine; Pituitary Gland; Procedures; Protease Inhibitor; Publishing; Rattus; Reagent; Regulation; Regulation of Exocytosis; Research Personnel; Role; Structure of superior cervical ganglion; Transfection; Variant; base; blood pressure regulation; cell preparation; chromogranin A (344-364); ganglion cell; programs; research study

Core F will provide sympathochromaffin cell cultures and related culture cell systems for investigators of projects of this program. Core F will prepare, on a routine basis, primary cultures that include sympathochromaffin cells from bovine adrenal medulla, superior cervical ganglion cells, and related cells. Neuroendocrine cell lines will be cultured and will consist of rat adrenal medullary PC12cells, and mouse pituitary AtT-20 cells, and other related cells required for experimental goals. This core will prepare, maintain, and grow cells for experiments supervised by project leaders. The uniformity of cells prepared with identical procedures for projects of this program will allow integration of scientific results with respect to the roles of multiple effectors that undergo exocytosis for the regulation of blood pressure. Core F will also provide cells treated with reagents that influence exocytosis of blood pressure regulators. Cells will undergo treatments with nicotine, forskolin, catestatin, NPY, protease inhibitors, and related reagents that modify functions of secreted neuroeffectors that influence blood pressure. Cells will also undergo uniform transfections with selected genes that possess variant SNP sequences related to human hypertension, based on molecular genetic data from the program. The cell culture core will promote synergistic interactions among projects and cores of this program project. Having well-defined secreting cell preparations that are uniform across several projects will enhance our ability to collaborate, integrate results in cohesive hypotheses and conclusions, and co-publish. These are important factors for evaluating the co-regulation of multiple sympathoeffector molecules that participate in blood pressure regulation.

CORE F将为交感神经嗜铬细胞培养和相关培养细胞系统的研究人员提供 这项计划的项目。核心F将在常规基础上准备主要培养物，包括 牛肾上腺髓质交感神经节细胞、颈上神经节细胞及相关细胞。 将培养神经内分泌细胞系，并将由大鼠肾上腺髓质PC12细胞和小鼠 垂体At-20细胞，以及实验目标所需的其他相关细胞。这个核心将会准备， 为项目负责人监督的实验维护和培养细胞。制备的电池的均匀度 该计划项目的相同程序将允许将科学结果与 多种效应因子在调节血压中的作用。核心F也将 提供经影响血压调节器胞吐作用的试剂处理的细胞。细胞将会经历 尼古丁、福斯可林、儿茶素、NPY、蛋白酶抑制剂和相关修饰试剂的治疗 影响血压的分泌型神经效应器的功能。细胞也将经历统一的 对具有与人类高血压相关的变异SNP序列的选定基因的转染，基于 来自该项目的分子遗传数据。细胞培养核心将促进 该计划项目的项目和核心。有明确的、均匀的分泌细胞制剂的 跨多个项目将增强我们的协作能力，将结果整合到连贯的假设中，并 结论，并共同发表。这些都是评价多种因素共同调控的重要因素 参与血压调节的交感神经效应分子。