Sympathochromaffin cell culture

交感嗜铬细胞培养

• 批准号: 7844961
• 负责人: Vivian Y. H Hook
• 金额: $ 24.11万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7844961
• 关键词: Adrenal Glands; Adrenal Medulla; Blood Pressure; Cattle; Cell Culture System; Cell Culture Techniques; Cell Line; Cells; Data; Exocytosis; Forskolin; Genes; Goals; Human; Hypertension; Molecular Genetics; Mus; Neuroeffector Junction; Neuroendocrine Cell; Nicotine; Pituitary Gland; Procedures; Protease Inhibitor; Publishing; Rattus; Reagent; Regulation; Regulation of Exocytosis; Research Personnel; Role; Structure of superior cervical ganglion; Transfection; Variant; base; blood pressure regulation; cell preparation; chromogranin A (344-364); ganglion cell; programs; research study

Core F will provide sympathochromaffin cell cultures and related culture cell systems for investigators of projects of this program. Core F will prepare, on a routine basis, primary cultures that include sympathochromaffin cells from bovine adrenal medulla, superior cervical ganglion cells, and related cells. Neuroendocrine cell lines will be cultured and will consist of rat adrenal medullary PC12cells, and mouse pituitary AtT-20 cells, and other related cells required for experimental goals. This core will prepare, maintain, and grow cells for experiments supervised by project leaders. The uniformity of cells prepared with identical procedures for projects of this program will allow integration of scientific results with respect to the roles of multiple effectors that undergo exocytosis for the regulation of blood pressure. Core F will also provide cells treated with reagents that influence exocytosis of blood pressure regulators. Cells will undergo treatments with nicotine, forskolin, catestatin, NPY, protease inhibitors, and related reagents that modify functions of secreted neuroeffectors that influence blood pressure. Cells will also undergo uniform transfections with selected genes that possess variant SNP sequences related to human hypertension, based on molecular genetic data from the program. The cell culture core will promote synergistic interactions among projects and cores of this program project. Having well-defined secreting cell preparations that are uniform across several projects will enhance our ability to collaborate, integrate results in cohesive hypotheses and conclusions, and co-publish. These are important factors for evaluating the co-regulation of multiple sympathoeffector molecules that participate in blood pressure regulation.

核心F将为研究人员提供交感神经菌细胞培养物和相关培养细胞系统 该计划的项目。核心F将在日常基础上准备，包括 来自牛肾上腺髓质，上颈神经节细胞和相关细胞的交感神经菌细胞。 神经内分泌细胞系将被培养，并由大鼠肾上腺髓质PC12细胞和小鼠组成 垂体ATT-20细胞和实验目标所需的其他相关细胞。这个核心将准备， 维护和生长细胞以进行项目负责人监督的实验。用的细胞均匀性 该计划项目的相同程序将允许就科学结果整合 多种效应子的作用，这些效应子在调节血压方面患有胞吐作用。核心F也将 提供用影响血压调节剂胞吐作用的试剂处理的细胞。细胞将经历 尼古丁，福斯科林，catestatin，npy，蛋白酶抑制剂和相关试剂的治疗 会影响血压的分泌神经反应器的功能。细胞也会均匀 基于具有与人类高血压相关的变异SNP序列的选定基因的转染，基于 该程序的分子遗传数据。细胞培养核心将促进协同互动 该计划项目的项目和核心。具有明确定义的分泌细胞制剂 在几个项目中 结论和共同出版。这些是评估多个共同调节的重要因素 参与血压调节的交感神经分子。