Integrated DNA Sequencing System

描述(由申请者提供)：NIH共享的目标 仪器应用是建立一个集成的、最先进的、 巴克研究所的DNA测序核心系统，以促进正在进行的 衰老及衰老相关分子机制的未来科学研究 疾病。最新技术开发了新的毛细管电泳法 测序仪器允许高质量、快速的DNA测序 以完全自动化的格式实现，使用敏感的荧光 染料终止剂。ABI 3100遗传分析仪系统的这些功能 毛细管电泳法具有传统平板凝胶无法比拟的优点 大多数其他DNA测序法使用的染料终止子DNA测序法 设施。毛细管制备、样品加载和 电泳法显著提高了结果的清晰度和一致性； 自动格式化避免了手动平板凝胶出现的操作员错误 常规DNA测序仪的格式。一体式DNA测序系统 将包括快速的8909寡核苷酸合成器，用于合成 大量DNA测序所需的引物；该装置还具有 合成多肽核酸(PNA)的独特能力 尤其对反义基因的表达以及合成 修饰和未修饰的寡核苷酸。这个集成的DNA测序系统 将加强正在进行的由NIH资助的研究(以及由其他来源资助的研究) 对于巴克研究所的主要研究人员来说，将补充现有的 基因组学、蛋白质组学、形态和细胞生物学的核心，并将促进 研究所研究人员之间的合作。

DESCRIPTION (provided by applicant): The goal of this NIH shared instrumentation application is to establish an integrated, state-of-the-art, DNA sequencing core system at the Buck Institute to facilitate ongoing and future scientific research on the molecular mechanisms of aging and age-related diseases. The latest technology has developed new capillary electrophoresis sequencing instrumentation to allow high quality, rapid DNA sequencing to be achieved in a completely automated format that utilizes sensitive fluorescent dye terminators. These features of the ABI 3100 Genetic Analyzer System for capillary electrophoresis system are advantageous over conventional slab gel dye terminator DNA sequencing that is used by most other DNA sequencing facilities. The automation of capillary preparation, sample loading, and electrophoresis significantly improves clarity and consistency of results; the automated format avoids operator errors that occur with the manual slab gel format of conventional DNA sequencers. The integrated DNA sequencing system will include the Expedite 8909 oligonucleotide synthesizer for synthesis of primers needed for the high volume of DNA sequencing; this unit also has the unique capability for synthesizing peptide nucleic acids (PNA) that are especially effective for antisense gene expression, as well as synthesis of modified and unmodified oligonucleotides. This integrated DNA sequencing system will enhance ongoing NIH-funded research (and research funded by other sources) for principal investigators at the Buck Institute, will complement existing cores in genomics, proteomics, and morphology and cell biology, and will foster collaborations among investigators at the Institute.