Core--Microsurgery

核心--显微外科

• 批准号: 7924683
• 负责人: George Tellides
• 金额: $ 21.53万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7924683
• 关键词: Adoptive Transfer; Animals; Aorta; Arterial Injury; Arteries; Arteriosclerosis; Biology; Blood Vessels; Cells; Chimera organism; Chronic; Complex; Coronary artery; Data; Ensure; Experimental Models; Genetic Models; Human; Human Resources; Immunodeficient Mouse; Interferon Type II; Leukocytes; Methodology; Methods; Microsurgery; Modeling; Mus; Play; Quality Control; Rag1 Mouse; Rattus; Research Personnel; Resources; Role; Techniques; Training; Transplantation; Universities; Vascular Graft; cytokine; experience; human tissue; improved; in vivo Model; mutant; programs; reconstitution; skills

The Microsurgery Core will provide expertise and quality control over animal transplantation models for Projects by Tellides, Pober, Min, and Bender of the program. The core unit will serve as a central resource to utilize the human artery transplantation models in immunodeficient mouse chimeras that have been developed at Yale University. Double-mutant severe combined immunodeficient (SCID)/beige mice are grafted with human or synthetic arteries and are subsequently immunologically reconstituted with an adoptive transfer of human leukocytes and/or are treated with human cytokines, such as the species-specific Th1 factor, interferon-gamma (IFN-y). The interactions of the leukocytes or cytokines with the graft vascular cells results in immunemediated arterial injury in a surrogate human experimental model. Additionally, various mouse recipient strains are grafted with mouse aorta segments to take advantage of the power of murine genetic models to supplement the human tissue data. The Microsurgery Core will also develop and adapt the artery graft models according to Project requirements, e.g. developing a Rag1 mutant rat recipient to study remodeling in larger primary branches of human epicardial coronary arteries. All four projects will be supported by the Microsurgery Core. The aims of the Microsurgery Core are:1) to provide the complex small animal transplantation models to the Program investigators; 2) to develop new methods for improving or adapting the in vivo models; and 3) to provide a microsurgery training resource for investigators in vascular and transplantation biology. The methodology of the human artery-SCID/beige mouse transplantation model is relatively complex and requires shared facilities and special skills. The Microsurgery Core personnel have extensive experience with the required techniques and their combined expertise is essential to ensure consistency of the models and an economy of scale. By providing the artery graft models to all of the projects, the Microsurgery Core will play a key role in this program.

显微外科核心将为以下动物移植模型提供专业知识和质量控制 项目由Tellides，Pober，Min和Bender主持。核心单元将作为中心资源，利用耶鲁大学开发的免疫缺陷小鼠嵌合体中的人类动脉移植模型 大学。将双突变严重联合免疫缺陷(SCID)/米色小鼠移植人或 并随后用过继转移的人 白细胞和/或用人类细胞因子治疗，如物种特异性Th1因子，干扰素-伽马 (干扰素-γ)。白细胞或细胞因子与移植物血管细胞的相互作用导致免疫调节 代用人体实验模型中的动脉损伤。此外，各种鼠标接收器 菌株被移植到小鼠的主动脉节段，以利用小鼠遗传模型的力量 补充人体组织数据。显微外科核心公司还将开发和改造动脉移植物 根据项目要求建立模型，例如开发Rag1突变大鼠受体以研究重塑 在人的心外膜冠状动脉的较大一级分支中。所有四个项目都将得到 显微外科核心。显微外科核心的目标是：1)为复杂的小动物 向项目调查人员移植模型；2)开发改进或适应的新方法 活体模型；以及3)为血管和血管领域的研究人员提供显微外科培训资源 移植生物学。人动脉-SCID/褐鼠移植模型的方法学为 相对复杂，需要共享设施和特殊技能。显微外科核心人员拥有 在所需技术及其组合专业知识方面具有丰富的经验，这对于确保 模式的一致性和规模经济。通过将动脉移植模型提供给所有 项目中，显微外科核心将在这一计划中发挥关键作用。