Assay Implementation

我们的中心现在在开发基于细胞和珠粒的分析方面拥有广泛的经验 用于目标团队进度报告中总结的流式细胞仪。我们有13名经过实验室培训的资深员工 人员包括博士9人，硕士3人，本科1人。其中11名工作人员有使用流式细胞仪进行AD/A的经验 这些工作人员中有11人在HTS机器人方面有经验，其中2人曾在化学库工作。 自从我们的中心通过MLSCN将流式细胞术的HT多路复用引入科学界以来， 我们的团队成员支持拓展开发一条目标管道，该管道可以无限期地延伸到 未来。斯克拉尔、普罗斯尼茨和爱德华兹已经做了60场外展演讲，以突出路线图目标和 机遇。几乎所有的检测都涉及目标提供商和我们中心之间的合作。 在迄今提交的化验中，除了VLA-4和Quorom Sensing化验外，几乎所有的化验都需要 发展阶段。

Our Center now has a broad range of experience in developing cell and bead based assays for the flow cytometer As summarized in target team progress report. We have 13 laboratory trained senior staff including 9PhDs, 3 MSs, and 1 BS. Eleven of these staff have experience in AD/A with flow cytometry and 11 of these staff have experience in HTS robotics including 2 who have worked with chemical libraries. Since HT multiplexing by flow cytometry was introduced to the scientific community by our Center via MLSCN, our team members have supported outreach to develop a target pipeline that can extend indefinitely into the future. Sklar, Prossnitz, and Edwards have given >60 outreach presentations to highlight Roadmap targets and opportunities. Nearly all of the assays involve collaborations between the target provider and our Center. Among the assays submitted so far, essentially all but the VLA-4 and Quorom Sensing assays required a development phase.