Role of Intronic Variants Affecting Splicing in Juvenile Myoclonic Epilepsy

影响剪接的内含子变异在青少年肌阵挛性癫痫中的作用

基本信息

  • 批准号:
    7713471
  • 负责人:
  • 金额:
    $ 16.04万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2009
  • 资助国家:
    美国
  • 起止时间:
    2009-09-30 至 2011-08-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Juvenile Myoclonic Epilepsy (JME) is among the most common types of syndromes of Idiopathic Generalized Epilepsy (IGE) and has a complex genetic inheritance. Although several putative JME susceptibility genes have been identified, the functions of these genes in the brain and their roles in the pathogenesis of seizures are unknown. Linkage and association analysis led us to the bromodomain-containing gene BRD2. BRD2 is a member of BET subfamily of double bromodomain-containing genes implicated in regulating gene expression. We have shown that BRD2 is expressed in developing and adult brain, and recently, that Brd2-null embryos die at mid-gestation with central nervous system malformations. Further, our preliminary data show that heterozygous Brd2 mice exhibit a reduction in the number of GABAergic interneurons in the frontal cortex and, strikingly, heterozygous Brd2 mice exhibit an enhanced sensitivity to chemically-induced seizures. Our working hypothesis is that mis-expression of BRD2 contributes to seizure susceptibility. We have also discovered that intron 2 of BRD2 contains a highly conserved, alternatively spliced exon, which introduces a premature termination codon and we have identified polymorphisms in the lengths of CA-repeats in intron 2 that are associated with JME. Variation in the length of CA-repeats has been shown to affect alternative splicing events in other experimental models. We now wish to test the hypothesis that the polymorphisms in intron 2, in particular variants in CA repeat-length that we have identified in patient DNA that associate with JME, affect splicing of the alternative exon and the levels of the corresponding transcripts, and hence, function of BRD2 products. In Aim 1, we will initially examine the effect of variable CA-repeat lengths on the relative levels of the two alternatively spliced BRD2 transcripts by transient expression of 'mini-gene' constructs containing [exon 2]-[intron 2]-[exon3] of human BRD2 with varying CA-repeat lengths and measuring the ratio of the resulting two transcripts. In Aim 2, we will begin to elucidate the functional significance of the alternatively spliced transcripts by determining whether the alternative transcripts are translated and if so, whether the proteins made affect homodimerization or sub-cellular localization. PUBLIC HEALTH RELEVANCE: Juvenile Myoclonic Epilepsy (JME) is among the most common types of syndromes of Idiopathic Generalized Epilepsy (IGE) and is characterized by adolescent onset and life-long affliction, requiring continual medication to suppress seizures. The proposed experiments will demonstrate whether the JME- associated variants in intron 2 of BRD2 play a direct role in the frequency of alternative splicing, and further, will provide insight into the possible physiological significance of the alternative transcripts, outcomes that are highly relevant to the stated goals of the RFA and the R03 funding mechanism.
描述(由申请人提供):青少年肌阵挛性癫痫(JME)是特发性全身性癫痫(IGE)最常见的综合征类型之一,具有复杂的遗传性。虽然已经确定了几个假定的JME易感基因,这些基因在大脑中的功能和癫痫发作的发病机制中的作用是未知的。连锁和关联分析使我们找到了含溴结构域的基因BRD 2。BRD 2是涉及调节基因表达的含双溴结构域基因的BET亚家族的成员。我们已经表明,BRD 2在发育和成人大脑中表达,最近,Brd 2无效的胚胎在妊娠中期死于中枢神经系统畸形。此外,我们的初步数据显示,杂合Brd 2小鼠表现出额叶皮层中GABA能中间神经元数量的减少,并且引人注目的是,杂合Brd 2小鼠表现出对化学诱导的癫痫发作的敏感性增强。我们的工作假设是BRD 2的错误表达有助于癫痫发作的易感性。我们还发现,内含子2的BRD 2包含一个高度保守的,选择性剪接的外显子,它引入了一个提前终止密码子,我们已经确定了多态性的长度在内含子2中的CA-重复与JME。在其他实验模型中,CA重复序列长度的变化已被证明会影响选择性剪接事件。我们现在希望检验这样的假设,即内含子2中的多态性,特别是我们在患者DNA中鉴定的与JME相关的CA重复长度的变体,影响选择性外显子的剪接和相应转录物的水平,从而影响BRD 2产物的功能。在目标1中,我们将首先通过瞬时表达含有具有不同CA重复序列长度的人BRD 2的[外显子2]-[内含子2]-[外显子3]的“迷你基因”构建体并测量所得两种转录物的比率来检查可变CA重复序列长度对两种选择性剪接的BRD 2转录物的相对水平的影响。在目标2中,我们将开始阐明选择性剪接转录本的功能意义,通过确定选择性转录本是否被翻译,如果是,所产生的蛋白质是否影响同源二聚化或亚细胞定位。 公共卫生关系:青少年肌阵挛性癫痫(JME)是特发性全身性癫痫(IGE)最常见的综合征之一,其特征是青少年发病和终身患病,需要持续药物治疗以抑制癫痫发作。所提出的实验将证明BRD 2的内含子2中的JME相关变体是否在选择性剪接的频率中发挥直接作用,并且进一步地,将提供对选择性转录物的可能生理意义的洞察,结果与RFA和R 03资助机制的所述目标高度相关。

项目成果

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DEBRA J. WOLGEMUTH其他文献

Recombination intermediates (reply)
重组中间体(回复)
  • DOI:
    10.1038/289097d0
  • 发表时间:
    1981-01-01
  • 期刊:
  • 影响因子:
    48.500
  • 作者:
    DEBRA J. WOLGEMUTH;MING-TA Hsu
  • 通讯作者:
    MING-TA Hsu

DEBRA J. WOLGEMUTH的其他文献

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{{ truncateString('DEBRA J. WOLGEMUTH', 18)}}的其他基金

Function of the Bromodomain Protein Brdt in Spermatogenesis
Bromodomain 蛋白 Brdt 在精子发生中的功能
  • 批准号:
    7810433
  • 财政年份:
    2010
  • 资助金额:
    $ 16.04万
  • 项目类别:
Retinoid receptor antagonists as novel male contraceptives
类维生素A受体拮抗剂作为新型男性避孕药
  • 批准号:
    8056646
  • 财政年份:
    2009
  • 资助金额:
    $ 16.04万
  • 项目类别:
Role of Intronic Variants Affecting Splicing in Juvenile Myoclonic Epilepsy
影响剪接的内含子变异在青少年肌阵挛性癫痫中的作用
  • 批准号:
    7941948
  • 财政年份:
    2009
  • 资助金额:
    $ 16.04万
  • 项目类别:
Retinoid receptor antagonists as novel male contraceptives
类维生素A受体拮抗剂作为新型男性避孕药
  • 批准号:
    7626133
  • 财政年份:
    2009
  • 资助金额:
    $ 16.04万
  • 项目类别:
Retinoid Receptor Antagonists as Novel Male Contraceptives
类维生素A受体拮抗剂作为新型男性避孕药
  • 批准号:
    8726695
  • 财政年份:
    2009
  • 资助金额:
    $ 16.04万
  • 项目类别:
Retinoid Receptor Antagonists as Novel Male Contraceptives
类维生素A受体拮抗剂作为新型男性避孕药
  • 批准号:
    9055741
  • 财政年份:
    2009
  • 资助金额:
    $ 16.04万
  • 项目类别:
Role of Intronic Variants Affecting Splicing in Juvenile Myoclonic Epilepsy
影响剪接的内含子变异在青少年肌阵挛性癫痫中的作用
  • 批准号:
    8133221
  • 财政年份:
    2009
  • 资助金额:
    $ 16.04万
  • 项目类别:
Retinoid receptor antagonists as novel male contraceptives
类维生素A受体拮抗剂作为新型男性避孕药
  • 批准号:
    8427386
  • 财政年份:
    2009
  • 资助金额:
    $ 16.04万
  • 项目类别:
Retinoid receptor antagonists as novel male contraceptives
类维生素A受体拮抗剂作为新型男性避孕药
  • 批准号:
    8228075
  • 财政年份:
    2009
  • 资助金额:
    $ 16.04万
  • 项目类别:
Retinoid receptor antagonists as novel male contraceptives
类维生素A受体拮抗剂作为新型男性避孕药
  • 批准号:
    8537001
  • 财政年份:
    2009
  • 资助金额:
    $ 16.04万
  • 项目类别:

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