La Jolla Interdisciplinary Neuroscience Center Cores - Electro Core BU

电生理学核心 一、主要目标和新方向 电生理学核心将是一个新的设施。目前，还没有统一的电生理学 连接La Jolla Torrey Pines台地神经科学家的核心设施。因此，这 电生理学设施将成为促进神经科学家合作的新核心 在圣地亚哥地区。 了解神经系统是如何工作的需要电生理技术来获得 对突触活动是如何被调节和调节的洞察。这是电生理学的核心 神经科学蓝图计划旨在将电生理学作为研究工具提供给 托里·松树台地上的众多神经学家。在过去的几年里，联合- 这一核心的董事斯蒂芬·F·海尔内曼博士和查尔斯·F·史蒂文斯博士使主要 对了解两个主要的兴奋性发射系统的功能的贡献 烟碱型乙酰胆碱和谷氨酸受体系统。他们的工作提供了对 突触传递和突触可塑性的机制。两位研究人员都做出了贡献 他们的知识和努力与神经学家的一系列合作没有直接的 电生理分析的专业知识或实验装置。虽然这些合作 取得了重大的研究进展，调查人员总是依赖于 主办实验室可以在仪器可用性方面容纳更多的实验，并 人事部。随着发育和系统对电生理学的需求不断增加 神经生物学家说，这是及时填补电生理学的额外能力的需要。至 实现这一目标，并进一步增加神经科学家之间在 Torrey Pines Mesa，一个新的电生理学核心设施将作为 神经科学蓝图计划。史蒂芬·海内曼博士和查尔斯·史蒂文斯博士将担任 联席董事致力于这一核心，并致力于为电生理的设计做出贡献 核心用户的实验。电生理学核心有两个目标：第一，提供 索尔克大学神经科学家用于电生理实验的专业知识和仪器 加州大学伯纳姆分校生物医学研究所(BIMR)和斯克里普斯研究所 第二，通过两个机构之间的对话与合作，加强神经科学 这些研究所的科学家。 电生理学核心增加了一个新的研究维度，将提供工具和 功能和活动依赖型研究的技术诀窍，旨在获得 神经元发生的固有或对外界反应的活动依赖性变化 信号。主要使用者提出的研究项目要求研究神经活动。 脑片和/或原代神经元培养。此外，这个核心将与核心之间的相位，以 病毒载体/siRNA和分子成像核心检测荧光标记的野生型和 突变蛋白在脑片和原代细胞中异位表达后在神经元功能中的作用。

ELECTROPHYSIOLOGY CORE 1. MAIN OBJECTIVES AND NEW DIRECTIONS The Electrophysiology Core will be a new facility. Currently, there is no unified Electrophysiology Core Facility to link neuroscientists on the La Jolla Torrey Pines Mesa. Therefore, this Electrophysiology facility will constitute a new Core to foster collaborations among neuroscientists in the San Diego area. Understanding how the nervous system works requires electrophysiological techniques to gain insights into how synaptic activity is regulated and modulated. The Electrophysiology Core of this Neuroscience Blueprint Initiative aims to make available electrophysiology as a research tool to a wide number of neuroscientists on the Torrey Pines Mesa. In the past several years, the Co- Directors of this Core, Dr. Stephen F. Helnemann and Dr. Charles F. Stevens made major contributions to understanding the functions of the two major excitatory transmitter systems, the nicotinic acetylcholine and glutamate receptor systems. Their work provided major insights into mechanisms of synaptic transmission and synaptic plasticity. Both researchers have contributed their knowledge and effort to a series of collaborations with neuroscientists who do not have direct expertise or the experimental set-up for electrophysiological analyses. While these collaborations accomplished significant research advancements, investigators always depended on whether the host laboratory could accommodate additional experiments in terms of instrument availability and personnel. With the increasing need for electrophysiology by developmental and systems neurobiologists, it is timely to fill the need for additional capacity in electrophysiology. To accomplish that and to further increase collaborative ventures between neuroscientists on the Torrey Pines Mesa, a new core facility for electrophysiology will be created as part of the Neuroscience Blueprint Initiative. Dr. Stephen Heinemann and Dr. Charles Stevens will serve as Co-Directors for this core and are committed to contribute to the design of electrophysiological experiments by the core users. The Electrophysiology Core has two goals: First, to provide expertise and instrumentation for electrophysiological experiments by neuroscientists at the Salk Institute, UCSD, Burnham Institute for Biomedical Research (BIMR) and The Scripps Research Institute; and second, to enhance neuroscience through dialogue and collaboration between the scientists of these institutes. The Electrophysiology Core adds a new research dimension that will provide the tools and know-how for functional and activity-dependent studies designed to acquire knowledge of the activity-dependent changes occurring in neurons either intrinsically or in response to external signals. The research projects proposed by the major users require studies of neuronal activity in brain slices and/or primary neuron cultures. Moreover, this core will inter-phase with the cores for Viral Vectors/siRNA and for Molecular Imaging cores to test fluorescently tagged wild type and mutant proteins in neuronal functions after ectopic expression in brain slices and primary cells.