La Jolla Interdisciplinary Neuroscience Center Cores - Molecular Imaging Hist Cor

细胞成像与组织学核心 一、主要目标和新方向 根据NIH神经科学蓝图核心中心的拨款，细胞成像/组织学核心将是 作为伯纳姆医学研究所现有癌症中心核心的扩建而建 研究(BIMR)。正如在这项拨款申请的其他地方所描述的那样，从历史上看，癌症中心的核心 不允许在La Jolla Torrey Pines台地接触神经科学家。这项神经科学 中心赠款将提供平等的机会，并促进新的神经科学专用成像工具和 神经科学界的组织学相关应用。核心还将推进 分子和细胞对神经发育、功能和病理的了解，并作为 圣地亚哥神经科学界合作的发起人。 目前的神经科学研究广泛使用遗传操作来解决这一问题 在模型生物体和神经培养中的特定分子。分子的观察 在这些系统中实施的变化严重依赖于组织学/免疫组织学和 先进的分子成像技术。神经成像和组织学核心的使命是 为Torrey上的神经科学社区提供最先进的成像和组织学工具 松树台地。目前，索尔克研究所和BIMR的神经科学家只能有限地获得 神经系统神经成像和组织学方面的专业知识和仪器。《Salk》 该研究所使用标准的单光子共聚焦显微镜(徕卡TCS SP2 AOBS)在共享的 适用于分子成像的碱基，被索尔克研究人员广泛使用。然而， 这种仪器的能力是有限的，特别是当涉及到细胞或 厚组织制剂中的分子事件，或活体组织制剂。伯纳姆研究所的 现有的细胞成像和组织学设施在其装备良好的核心，即 多光子共聚焦成像的仪器、专业知识和能力将适应这一点 空虚。现有的Salk和BIMR设施都不提供专门针对以下方面的服务 神经科学的应用。目前的组织学BIMR服务非常熟悉准备工作 和分析肿瘤和大型器官，但不能适应面向分析的任务 神经系统复杂结构中的特定结构。这件事的目标是 神经科学蓝图倡议就是要克服这些限制，建立一个开放获取核心 托里松树台地上的神经科学家设施。 伯纳姆研究所的癌症中心细胞成像设备经历了显著的 在过去的一年里进行了扩张，并投资了三台新的共焦显微镜作为其 机构承诺扩大使用和能力。有了这些新的仪器，成像 BIMR的能力得到了显著改进，现在还开放了更多的成像能力。近期 随着分子成像技术的发展，分子成像技术经历了显著的进步 加州大学圣地亚哥分校的罗杰·钱存训博士致力于荧光染料的研究，这种染料可以追踪活细胞内的分子。Dr。 钱存训将把他开发的成像工具带到这个神经科学蓝图核心和 以联席主任的身份参与对神经系统分子的“监视”。因此，用户将 通过这一核心，您可以轻松访问钱博士开发的工具。 对于核心的组织学部分，在解剖学和组织学方面的专门现场专门知识 神经系统将被添加到现有的癌症中心BIMR组织设施中。核心意志 适应大脑切片的准备，以查看 发育中的成人神经系统。此外，Core将使用最先进的激光器 微切割系统，将允许切除特定的细胞或核以用于微阵列和蛋白质组 侧写。因此，成像/组织学核心将与基因分析和蛋白质组学相互作用 此应用程序的核心。 电子显微镜(EM)不包括在本影像/组织学核心中，作为神经科学 研究人员将通过以下途径获得优秀的神经系统超微结构分析 在这笔赠款中，由Eliezer Masliah博士指导的神经病理学核心(见Neuroology Core 此应用程序中的描述)。通过神经病理学核心，神经科学家在Torrey Pines Mesa还优先进入国家显微镜和成像中心 加州大学圣迭戈分校的Mark Ellisman博士领导的研究(NCMIR)设施。NCMIR设施支持 扫描、透射式和高压电子显微镜。 细胞成像和组织学核心的任务是扩展分子和细胞成像 为Torrey Pines台地的神经科学界提供能力和组织学服务。这个 主要核心目标是： 1)结合分子探针提供高分辨率显微技术，以获得 关于细胞内分子的位置、运动和活动的信息； 2)提供组织学服务，以揭示海马区的解剖结构和细胞成分 用于发育和成人神经系统，以及用于基因阵列的细胞群的激光解剖 和蛋白质组分析。 3)提供咨询、培训用户并协助分析核心内产生的数据。 4)通过信息交流和跨阶段工作促进神经科学家之间的合作 与这一蓝图倡议的其他核心。

CELL IMAGING AND HISTOLOGY CORE 1. MAIN OBJECTIVES AND NEW DIRECTIONS Under the NIH Neuroscience Blueprint Core Center Grant, the Cell Imaging/Histology Core will be built as an expansion of an existing Cancer Center core at the Burnham Institute for Medical Research (BIMR). As described elsewhere in this grant application, historically, Cancer Center cores have not allowed access to neuroscientists on the La Jolla Torrey Pines Mesa. This Neuroscience Center Grant will provide equal access and foster novel Neuroscience-specific imaging tools and histology-related applications for the Neuroscience community. The Core will also advance the molecular and cellular understanding of neural development, function and pathology, and serve as an initiator for collaborations in the Neuroscience community throughout San Diego. Current neuroscience research widely employs genetic manipulations to address the workings of specific molecules in model organisms and neural cultures. The observation of the molecular changes implemented in these systems critically depends on histological/immunohistological and advanced molecular imaging techniques. The mission of the Neuro-lmaging and Histology Core is to provide state-of-the-art imaging and histological tools to the Neuroscience community on the Torrey Pines Mesa. Currently, Neuroscientists at the Salk Institute and BIMR have only limited access to expertise and instrumentation in neuronal imaging and histology of the nervous system. The Salk Institute operates a standard single photon confocal microscope (Leica TCS SP2 AOBS) on a shared basis that is suited for molecular imaging and extensively used by Salk researchers. However, the capabilities of this instrument are limited, in particular when it comes to imaging of cellular or molecular events in thick tissue preparations, or to live tissue preparations. The Burnham Institute's existing Cell Imaging and Histology Facility has available within its well-equipped Core, the instrumentation, expertise and capacity for multiphoton, confocal imaging that will accommodate this void. Neither the existing Salk nor the BIMR facilities offer services geared specifically to Neuroscience applications. The current BIMR service for histology is well versed for the preparation and analysis of tumors and large organs, but cannot accommodate tasks geared towards analyzing specific structures within the complex architecture of the nervous system. The goal for this Neuroscience Blueprint Initiative is to overcome these limitations and establish an open access Core Facility for neuroscientists on the Torrey Pines Mesa. The Burnham Institute's Cancer Center Cell Imaging Facility has experienced significant expansion during the past year and invested in three new confocal microscopes as part of its institutional commitment to expand use and capabilities. With these new instruments, the imaging capabilities at BIMR are significantly improved and additional imaging capacity is now open. Recent techniques in molecular imaging have experienced significant advancement with the development by Dr. Roger Tsien of UCSD of fluorescent dyes that allow the tracing of molecules within living cell. Dr. Tsien will bring the imaging tools he has developed to this Neuroscience Blueprint Core and participate in "spying" on the molecules of the nervous system as the Co-Director. Thus, users will gain easy access to the tools developed by Dr. Tsien through this Core. For the histology section of the Core, specialized on-site expertise in the anatomy and histology of the nervous system will be added to the existing Cancer Center BIMR Histology facility. The Core will accommodate the preparation of brain sections to view specified anatomical structures in the developing and adult nervous system. Moreover, the Core will operate a state-of-the-art laser microdissection system that will allow excising specified cells or nuclei for microarray and proteomic profiling. Thus, the Imaging/Histology Core will interphase with the Gene Analysis and the Proteomics Cores of this application. Electron microscopy (EM) is not included in this Imaging/Histology Core as Neuroscience researchers will have access to excellent ultrastructural analyses of the nervous system through the Neuropathology Core in this grant, which is directed by Dr. Eliezer Masliah (see Neuropathology Core Description in this application). Through the Neuropathology Core, Neuroscientists on the Torrey Pines Mesa also have priority access to the National Center for Microscopy and Imaging Research (NCMIR) Facility directed by Dr. Mark Ellisman of UCSD. The NCMIR Facility supports scanning-, transmitted-, and high voltage electron microscopy. The mission for the Cell Imaging and Histology Core is to extend molecular and cellular imaging capabilities and histology services to the Neuroscience community on the Torrey Pines Mesa. The main Core objectives are: 1) Provide high-resolution microscopic techniques in combination with molecular probes to obtain information about the location, movement, and activities of molecules within cells; 2) Provide histological services to reveal the anatomical structures and cellular components of the developing and adult nervous system, and for the laser dissection of cell populations for gene array and proteome analysis. 3) Offer consultation, train users and assist with the analysis of the data generated within the Core. 4) Foster collaborations between neuroscientists through information exchange and interphase work with the other Cores of this Blueprint Initiative.