CATION AND LIGAND BINDING TO UNUSUAL DNA STRUCTURES

阳离子和配体与异常 DNA 结构的结合

• 批准号: 7955477
• 负责人: RICHARD H SHAFER
• 金额: $ 0.88万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955477
• 关键词: Binding; Cations; Chimera organism; Circular Dichroism; Complex; Computer Retrieval of Information on Scientific Projects Database; Computing Methodologies; DNA; DNA Structure; Funding; Grant; Imagery; Informatics; Institution; Ligand Binding; Ligands; Nuclear Magnetic Resonance; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; biocomputing; programs; quadruplex DNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are investigating the details of cation binding to DNA quadruplexes and DNA-ligand complexes as determined by Circular dichroism, Nuclear Magnetic Resonance (NMR), as well as computational methods. The analysis of these structures requires use of Chimera, MidasPlus, and Sparky, (all developed within the RBVI) as well as other programs. Chimera is also used to help visualize results and obtain otherwise had to obtain views and structural comparisons.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们正在研究通过圆二色性、核磁共振 (NMR) 以及计算方法确定的阳离子与 DNA 四链体和 DNA-配体复合物结合的细节。 这些结构的分析需要使用 Chimera、MidasPlus 和 Sparky（全部在 RBVI 内开发）以及其他程序。 Chimera 还用于帮助可视化结果并获得否则必须获得的视图和结构比较。