CATION AND LIGAND BINDING TO UNUSUAL DNA STRUCTURES

阳离子和配体与异常 DNA 结构的结合

• 批准号: 8363591
• 负责人: RICHARD H SHAFER
• 金额: $ 1.02万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363591
• 关键词: Binding; Cations; Chimera organism; Circular Dichroism; Complex; Computing Methodologies; DNA; DNA Structure; Funding; Grant; Imagery; Informatics; Ligand Binding; Ligands; National Center for Research Resources; Nuclear Magnetic Resonance; Principal Investigator; Research; Research Infrastructure; Resources; Source; Structure; United States National Institutes of Health; biocomputing; cost; programs; quadruplex DNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We are investigating the details of cation binding to DNA quadruplexes and DNA-ligand complexes as determined by Circular dichroism, Nuclear Magnetic Resonance (NMR), as well as computational methods. The analysis of these structures requires use of Chimera, MidasPlus, and Sparky, (all developed within the RBVI) as well as other programs. Chimera is also used to help visualize results and to obtain views and structural comparisons.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们正在研究阳离子结合的DNA四链体和DNA-配体复合物的细节，确定通过圆二色谱，核磁共振（NMR），以及计算方法。 这些结构的分析需要使用Chimera、MidasPlus和Sparky（均在RBVI内开发）以及其他程序。 Chimera还用于帮助可视化结果，并获得视图和结构比较。