USING ANNOTATED PEPTIDE MASS SPECTRUM LIBRARIES FOR PROTEIN IDENTIFICATION

使用带注释的肽质谱库进行蛋白质鉴定

• 批准号: 7954106
• 负责人: RONALD C. BEAVIS
• 金额: $ 0.24万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954106
• 关键词: Charge; Computer Retrieval of Information on Scientific Projects Database; Computer software; Databases; Funding; Grant; Homo sapiens; House mice; Institution; Ions; Libraries; Mass Spectrum Analysis; Modification; Parents; Peptides; Proteins; Proteome; Research; Research Personnel; Resources; Saccharomyces cerevisiae; Source; System; United States National Institutes of Health; base; macromolecule; protein aminoacid sequence

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A system for creating a library of tandem mass spectra annotated with corresponding peptide sequences was described. This system was based on the annotated spectra currently available in the Global Proteome Machine Database (GPMDB). The library spectra were created by averaging together spectra that were annotated with the same peptide sequence, sequence modifications, and parent ion charge. The library was constructed so that experimental peptide tandem mass spectra could be compared with those in the library, resulting in a peptide sequence identification based on scoring the similarity of the experimental spectrum with the contents of the library. A software implementation that performs this type of library search was constructed and successfully used to obtain sequence identifications. The annotated tandem mass spectrum libraries for the Homo sapiens, Mus musculus, and Saccharomyces cerevisiae proteomes and search software were made available for download and use by other groups.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 描述了用于创建用相应肽序列注释的串联质谱库的系统。该系统基于全球蛋白质组机器数据库（GPMDB）中当前可用的注释光谱。通过对用相同肽序列、序列修饰和母离子电荷注释的光谱进行平均来创建库光谱。构建文库后，可以将实验肽串联质谱与文库中的肽串联质谱进行比较，从而根据实验光谱与文库内容的相似性进行评分，从而进行肽序列鉴定。构建了执行此类库搜索的软件实现并成功用于获得序列识别。智人、小家鼠和酿酒酵母蛋白质组的注释串联质谱库和搜索软件可供其他团体下载和使用。