USING ANNOTATED PEPTIDE MASS SPECTRUM LIBRARIES FOR PROTEIN IDENTIFICATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 描述了一种用于创建用对应的肽序列注释的串联质谱库的系统。该系统基于全球蛋白质组机器数据库(GPMDB)中目前可用的注释谱。文库光谱是通过将用相同的肽序列、序列修改和母体离子电荷注释的光谱平均在一起而创建的。构建了文库，以便将实验多肽串联质谱图与文库中的多肽串联质谱图进行比较，从而得到基于实验谱图与文库内容相似性的多肽序列鉴定。构建了执行这种类型的文库搜索的软件实现，并成功地用于获得序列鉴定。智人、小鼠和酿酒酵母蛋白质组的注释串联质谱库和搜索软件可供其他组下载和使用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A system for creating a library of tandem mass spectra annotated with corresponding peptide sequences was described. This system was based on the annotated spectra currently available in the Global Proteome Machine Database (GPMDB). The library spectra were created by averaging together spectra that were annotated with the same peptide sequence, sequence modifications, and parent ion charge. The library was constructed so that experimental peptide tandem mass spectra could be compared with those in the library, resulting in a peptide sequence identification based on scoring the similarity of the experimental spectrum with the contents of the library. A software implementation that performs this type of library search was constructed and successfully used to obtain sequence identifications. The annotated tandem mass spectrum libraries for the Homo sapiens, Mus musculus, and Saccharomyces cerevisiae proteomes and search software were made available for download and use by other groups.